Jean-Michel Girardet


– 2015: Currently assigned to UMR 1136 INRA/University of Lorraine IAM as Research Engineer.Manager of the ‘Structural and Functional Approaches of Cellular InterActions’ (ASIA) platform located at the Faculty of Sciences and Technologies:

– 1993-2014: Engineer in Food Biochemistry assigned to UR AFPA, USC INRA 340, University of Lorraine.


– 2012: H.D.R. (Qualified Research Supervisor) from the University of Lorraine, France: ‘Technofunctional and Biofunctional Properties of Milk Proteins and Peptides’.

– 1992: Ph.D. in Applied Biochemistry from the University of Nancy 1, France : ‘Component PP3 of Milk: Preparation, Origin, Study of its Glycan Moiety, Role in the Inhibition of Lipolysis’. (INRA-Region of Lorraine scholarship)


– Elected member of staff (CPE) at the University of Lorraine (ca. 3000 technicians and engineers concerned).

– Appointed as expert in juries in national competitions for the recruitment of staff.

– Elected member at the council of A2F scientific cluster of University of Lorraine.

– Elected member at the scientifc council of UMR IAM.



Narmuratova Z., Hentati F., Girardet J.M., Narmuratova M., & Cakir-Kiefer C. (2022). Equine lactoferrin: Antioxidant properties related to divalent metal chelation. LWT Food Science and Technology, 161, 113426.


El Hajj S., Sepúlveda-Rinconi T., Girardet J.M., Cakir-Kiefer C., Stefan L., Zapata-Montoya J.E., Boshi-Muller S., Gaucher C. & Canabady-Rochelle L. (2021) Electrically switchable nanolever technology for the screening of metal-chelating peptides in hydrolysates. Journal of Agricultural and Food Chemistry, 69, 8819-8827.

Perrot T., Schwartz M., Deroy A., Girardet J.M., Kohler A., Morel-Rouhier M., Favier F., Gelhaye E. & Didierjean C. (2021). Diversity of Omega Glutathione Transferases in mushroom-forming fungi revealed by phylogenetic, transcriptomic, biochemical and structural approaches. Fungal Genetics and Biology, 148, 103506.

Bchini R., Girardet J.M., Sormani R., Gelhaye E. & Morel-Rouhier M. (2021). Oxidized glutathione promotes association between Eukaryotic Translation Elongation Factor 1Bγ and Ure2p glutathione transferase from Phanerochaete chrysosporium. The FEBS Journal, 288, 2956-2969.

El Hajj S., Sepúlveda-Rinconi T., Selmeczi K., Paris C., Giraud T., Csire G., Stefan L., Girardet J.M., Desobry S., Bouhallab S., Muhr L., Gaucher C. & Canabady-Rochelle L. (2021) Chapter 19, Applications in Nutrition: Mineral-Binding. In Biologically Active Peptides 1st Ed.: From basic science to applications for human health (Fidel Toldrá and Jianping Wu, Eds.), pp 455-494, Elsevier. Academic Press (512 pages).


Roret, T., Alloing G., Girardet, J.M., Perrot T., Dhalleine, T., Couturier J., Frendo P., Didierjean C. & Rouhier N. (2020). Sinorhizobium meliloti YrbA binds divalent metal cations using two conserved histidines. Bioscience Reports, 40, BSR20202956.

Delannoy M., Girardet J.M., Djelti F., Yen F.T. & Cakir-Kiefer C. (2020). Affinity of chlordecone and chlordecol for human serum lipoproteins. Environmental Toxicology and Pharmacology, 80, 103486.

Vicente C.M., Girardet J.M., Hôtel L. & Aigle B. (2020). Molecular dynamics to elucidate the DNA-binding activity of AlpZ, a member of the gamma-butyrolactone receptor family in S. ambofaciens. Frontiers in Microbiology: Antimicrobials, Resistance and Chemotherapy, 11, 1255.

Sylvestre-Gonon E., Schwartz M., Girardet J.M., Hecker A. & Rouhier N. (2020). Is there a role for tau glutathione transferases in tetrapyrrole metabolism and retrograde signalling in plants? Philosophical Transactions of the Royal Society B, 375, 20190404.


Si Ahmed Zennia S., Mati A., Charron C., Cakir-Kiefer C., Kriznik A. & Girardet J.M.  (2019). Effect of nonenzymatic deamidation on the structure stability of Camelus dromedarius alpha-lactalbumin. Food Chemistry, 291, 207-213.


Perrot T., Schwartz M., Saiag F., Salzet G., Dumarcay S., Favier F., Gerardin P., Girardet J.M., Sormani R., Morel-Rouhier M., Amusant N., Didierjean C. & Gelhaye E. (2018). Fungal glutathione transferases as tools to explore the chemical diversity of Amazonian wood extractives. ACS Sustainable Chemistry & Engineering, 6, 13078-13085.

Mekhaneg B., Girardet J.M., Humbert G., Saulnier F., Poirson C. & Bellal M.-M. (2018). Physico-chemical characterization of a milk-clotting fraction extracted from turkey (Meleagris gallopavo) proventriculus. Innovative Food Science and Emerging Technologies, 48, 172-178.


Mati A., Senoussi-Ghezali C., Si Ahmed Zennia S., Almi-Sebbane D., El-Hatmi H. & Girardet J.M. (2017). Dromedary camel milk proteins, a source of peptides having biological activities – A review. International Dairy Journal, 73, 25-37.


Schwartz M., Didierjean C., Hecker A., Girardet J.M., Morel-Rouhier M., Gelhaye E. & Favier F. (2016). Crystal structure of Saccharomyces cerevisiae ECM4, a Xi-class glutathione transferase that reacts with glutathionyl-(hydro)quinones. PLOS One, 11, e0164678.

Lallement P.A., Roret T., Tsan P., Gualberto J.M., Girardet J.M., Didierjean C., Rouhier N. & Hecker A. (2016). Insights into ascorbate regeneration in plants: Investigating the redox and structural properties of dehydroascorbate reductases from Populus trichocarpaBiochemical Journal, 473, 717-731.

Guerin J., Kriznik A., Ramalanjaona N., Le Roux Y. & Girardet J.M. (2016). Interaction between dietary bioactive peptides of short length and bile salts in submicellar or micellar state. Food Chemistry, 209, 114-122.

El Hatmi H., Jard Z., Khorchani T., Jardin J., Poirson C., Perrin C., Cakir-Kiefer C. & Girardet J.M. (2016). Identification of bioactive peptides derived from caseins, Glycosylation-Dependent Cell Adhesion Molecule-1 (GlyCAM-1), and Peptidoglycan Recognition Protein-1 in fermented camel milk. International Dairy Journal56, 159-168.

BOOK CHAPTER 2016-2021:

El Hajj S., Sepúlveda-Rinconi T., Selmeczi K., Paris C., Giraud T., Csire G., Stefan L., Girardet J.M., Desobry S., Bouhallab S., Muhr L., Gaucher C. & Canabady-Rochelle L. (2021) Chapter 19, Applications in Nutrition: Mineral-Binding. In Biologically Active Peptides 1st Ed.: From basic science to applications for human health (Fidel Toldrá and Jianping Wu, Eds.), Elsevier. Academic Press. pp. 455-494. ISBN 9780128213896.


Sylvestre-Gonon É., Girardet J.M., Dubos C., Rouhier N., & Hecker A. Biochemical characterization of glutathione transferases potentially involved in the metabolism of coumarins in Arabidopsis thaliana during iron deficiency. EMBO Workshop ‘Thiol oxidation in toxicity and signalling’, Sant Feliu de Guixols, Spain, September, 17 – 21, 2017.

Roret T., Couturier J., Girardet J.M., Didierjean C., & Rouhier N. Structural characterization of BolA-metal complexes through Sinorhizobium meliloti YrbA. 7ème Congrès de l’Association Française de Cristallographie (AFC), Marseille, France,
4 – 7 juillet 2016.



Hafeez Z., Cakir-Kiefer C., Girardet J.M., Lecomte X., Paris C., Galia W., Dary A. & Miclo L. (2015). New Insights into the proteolytic system of Streptococcus thermophilus: Use of isracidin to characterize cell-associated extracellular peptidase activities. Journal of Agricultural and Food Chemistry, 63, 7522-7531.

Si Ahmed Zennia S., Mati A., Saulnier F., Verdier Y., Chiappetta G., Mulliert G., Miclo L., Vinh J. & Girardet J.M. (2015). Identification by FT-ICR-MS of Camelus dromedarius alpha-lactalbumin variants as the result of nonenzymatic deamidation of Asn-16 and Asn-45. Food Chemistry, 187, 305-313.

Canabady-Rochelle L.L.S., Harscoat-Schiavo C., Kessler V., Fournier F., Aymes A. & Girardet J.M. (2015). Determination of reducing power and metal chelating ability of antioxidant peptides: Revisited Methods. Food Chemistry, 183, 129-135.


Jrad Z., Girardet J.M., Adt I., Oulahal N., Degraeve P., Khorchani T. & El Hatmi H. (2014). Antioxidant activity of camel milk casein before and after in vitro simulated enzymatic digestion. Mljekarstvo, 64, 287-294.

Jrad Z., El Hatmi H., Adt I., Girardet J.M., Cakir-Kiefer C., Jardin J., Degraeve P., Khorchani T. & Oulahal N. (2014). Effect of digestive enzymes on antimicrobial, radical scavenging and angiotensin I-converting enzyme inhibitory activities of camel colostrum and milk proteins. Dairy Science and Technology94, 205-224.

Bunet R., Riclea R., Laureti L., Hôtel L., Paris C., Girardet J.M., Spiteller D., Dickschat J.S., Leblond P. & Aigle B. (2014). A single Sfp-type phosphopantetheinyl transferase plays a major role in the biosynthesis of PKS and NRPS derived metabolites in Streptomyces ambofaciens ATCC23877. PLOS One, 9, e87607.

El Hatmi H., Z. Jrad Z., Khorchani T., Dary A. & Girardet J.M. (2014). Fast protein liquid chromatography of camel alpha-lactalbumin fraction with radical scavenging activity. Emirates Journal of Food and Agriculture, 26, 309-316.


Zidane F., Zeder-Lutz G., Altschuh D., Girardet J.M., Miclo L., Corbier C. & Cakir-Kiefer C. (2013). Surface plasmon resonance analysis of the binding mechanism of pharmacological and peptidic inhibitors to human somatic angiotensin I-converting enzyme. Biochemistry52, 8722-8731.

Hafeez, Z., Cakir-Kiefer, C., Girardet, J.M., Jardin, J., Perrin, C., Dary, A., & Miclo, L. (2013). Hydrolysis of milk-derived bioactive peptides by cell-associated extracellular peptidases of Streptococcus thermophilusApplied Microbiology and Biotechnology, 97, 9787-9799.


Ahmad N., Girardet J.M., Akbar S., Lanhers M.-C., Paris C., Yen F.T. & Corbier C. (2012). Lactoferrin and its hydrolysate bind directly to the oleate-activated form of the lipolysis stimulated lipoprotein receptor. FEBS Journal, 279, 4361-4373.

Zidane F., Matéos A., Cakir-Kiefer C., Miclo L., Rahuel-Clermont S., Girardet J.M. & Corbier C. (2012). Binding of divalent metal ions to 1-25 beta-caseinophosphopeptide: an isothermal titration calorimetry study. Food Chemistry132, 391-398.


Sadat L., Cakir-Kiefer C., N’Negue M.A., Gaillard J.L., Girardet J.M. & Miclo, L. (2011). Isolation and identification of antioxidative peptides from bovine alpha-lactalbumin. International Dairy Journal, 21, 214-221.


Matéos A., Girardet J.M., Mollé D., Corbier C., Gaillard J.L. & Miclo L. (2010). Identification of phosphorylation sites of equine beta-casein isoforms. Rapid Communications in Mass Spectrometry, 24, 1533-1542.

Before 2010:

Matéos A., Miclo L., Mollé D., Dary A., Girardet J.M. & Gaillard J.L. (2009). Equine alphas1-casein : characterization of alternative splicing isoforms and determination of phosphorylation levels. Journal of Dairy Science, 92, 3604-3615.

Matéos A., Girardet J.M., Mollé D., Dary A., Miclo L. & Gaillard J.L. (2009). Two-dimensional cartography of equine beta-casein variants achieved by isolation of phosphorylation isoforms and control of the deamidation phenomenon. Journal of Dairy Science, 92, 2389-2399.

Miclo L., Girardet J.M., Egito A.S., Mollé D., Martin P. & Gaillard J.L. (2007). The primary structure of a low-Mr multi-phosphorylated variant of beta-casein in equine milk. Proteomics 7, 1327-1335.

Egito A.S., Girardet J.M., Laguna L.E., Poirson C., Mollé D., Miclo L., Humbert G. & Gaillard J.L. (2007). Milk-clotting activity of enzyme extracts from sunflower and albizia seeds and specific hydrolysis of kappa‑casein. International Dairy Journal, 17, 816-825.

El-Hatmi H., Girardet J.M., Gaillard J.L., Yahyaoui M.H. & Attia H. (2007). Characterization of whey proteins of camel (Camelus dromedarius) milk and colostrum. Small Ruminant Research , 70, 267-271.

Egito A.S., Rosinha G.M.S., Laguna L.E., Miclo L., Girardet J.M. & Gaillard J.L. (2006). [Fast electrophoretic detection method of adulteration of caprine milk by bovine milk]. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, 58, 932-939.

Girardet J.M., Miclo L., Florent S., Mollé D. & Gaillard J.L. (2006). Determination of the phosphorylation level and deamidation susceptibility of equine beta-casein. Proteomics, 6, 3707-3717.

N’Negue M.A., Miclo L., Girardet J.M., Campagna S., Mollé D. & Gaillard J.L. (2006). Proteolysis of bovine alpha-lactalbumin by thermolysin during thermal denaturation. International Dairy Journal, 16, 1157-1167.

Pang X., Aigle B., Girardet J.M., Mangenot S., Pernodet J.L., Decaris B. & Leblond P. (2004). Functional angucycline-like antibiotic gene cluster in the terminal inverted repeats of the Streptomyces ambofaciens linear chromosome. Antimicrobial Agents and Chemotherapy, 48, 575-588.

Nabhan M.A., Girardet J.M., Campagna S., Gaillard J.L. & Le Roux Y. (2004). Isolation and characterization of co-polymers of beta-lactoglobulin, alpha-lactalbumin, kappa-casein, and alphas1-casein generated by pressurization and thermal treatment of raw milk. Journal of Dairy Science, 87, 3614-3622.

Campagna S., Mathot A.G., Fleury Y., Girardet J.M. & Gaillard J.L. (2004). Antibacterial activity of lactophoricin, a synthetic 23-residues peptide derived from the sequence of bovine milk component-3 of proteose peptone. Journal of Dairy Science, 87, 1621-1626.

Girardet J.M., N’Negue M.A., Egito A.S., Campagna S., Lagrange A. & Gaillard J.L. (2004). Multiple forms for equine alpha‑lactalbumin: evidence of N-glycosylated and deamidated forms. International Dairy Journal, 14, 207-217.

Egito A.S., Girardet J.M., Poirson C., Mollé D., Humbert G., Miclo L. & Gaillard J.L. (2003). Action of plasmin on equine beta‑casein. International Dairy Journal, 13, 813-820.

Moussaoui F., Laurent F., Girardet J.M., Humbert G., Gaillard J.L. & Le Roux Y. (2003). Characterization and proteolytic origins of specific peptides appearing during lipopolysaccharide experimental mastitis. Journal of Dairy Science, 86, 1163-1170.

Gelhaye E., Rouhier N., Vlamis-Gardikas A., Girardet J.M., Sautière P.E., Sauzet M., Martin F. & Jacquot J.P. (2003). Identification and characterization of a third thioredoxin h in poplar. Plant Physiology and Biochemistry, 41, 629-635.

Egito A.S., Miclo L., Lopez C., Adam A., Girardet J.M. & Gaillard J.L. (2002). Separation and characterization of mares’ milk alphas1-, beta-, kappa-caseins, gamma-casein-like, and proteose peptone component 5-like peptides. Journal of Dairy Science, 85, 697-706.

Egito A.S., Haussard M., Poirson C., Girardet J.M., Miclo L. & Gaillard J.L. (2002) Angiotensin I-converting enzyme inhibitory properties of an equine tryptic digest and characterisation of some active peptides. In Peptides 2002 pp. 92-93 (Ettore Benedetti and Carlo Pedone, Eds.), Edizioni Ziino, Napoli, Italia.

Egito A.S., Girardet J.M., Miclo L., Mollé D., Humbert G. & Gaillard J.L. (2001). Susceptibility of equine kappa- and beta-caseins to hydrolysis by chymosin. International Dairy Journal, 11, 885-893.

Egito A.S., Girardet J.M., Miclo L. & Gaillard J.L. (2001). Highly sensitive periodic acid/Schiff detection of bovine milk glycoproteins electrotransferred after nondenaturing electrophoresis, urea electrophoresis, and isoelectric focusing. Le Lait, 81, 775-785.

Girardet J.M., Humbert G., Creusot N., Chardot V., Campagna S., Courthaudon J.L. & Gaillard J.L. (2001). Dilational rheology of mixed beta-casein/Tween 20 and beta-casein (f114-169)/Tween 20 films at oil-water interface. Journal of Colloid and Interface Science, 243, 515-522.

Girardet J.M., Debomy L., Courthaudon J.L., Miclo L., Humbert G. & Gaillard J.L. (2000). Viscoelastic properties of oil-water interfaces covered by bovine beta-casein tryptic peptides. Journal of Dairy Science, 83, 2410-2421.

Girardet J.M., Saulnier F., Gaillard J.L., Ramet J.P. & Humbert G. (2000). Camel (Camelus dromedarius) milk PP3: evidence for an insertion in the amino-terminal sequence of the camel milk whey protein. Biochemistry and Cell Biology, 78, 19-26.

Courthaudon J.L., Girardet J.M., Campagne S., Rouhier L.M., Campagna S., Lorient D. & Linden G. (1999). Surface active and emulsifying properties of casein micelles compared to those of sodium caseinate. International Dairy Journal, 9, 411-412.

Girardet J.M., Courthaudon J.L., Campagna S., Puyjalon V., Lorient D. & Linden G. (1999). Proteolysis of milk casein micelle and sodium caseinate in oil-in-water emulsion. International Dairy Journal, 9, 409-410.

Montagne L., Girardet J.M. & Humbert G. (1999). Amino-terminal sequencing of the ovine milk fat globule membrane protein butyrophilin. Le Lait, 79, 275-279.

Campagna S., Vitoux B., Humbert G., Girardet J.M., Linden G., Haertle T. & Gaillard J.L. (1998). Conformational studies of a synthetic peptide from the putative lipid-binding domain of bovine milk component PP3. Journal of Dairy Science, 81, 3139-3148.

Girardet J.M., Saulnier F., Linden G. & Humbert G. (1998). Rapid separation of bovine whey proteins by membrane convective liquid chromatography, perfusion chromatography, continuous bed chromatography, and capillary electrophoresis. Le Lait, 78, 391-400.

Coddeville B., Girardet J.M., Plancke Y., Campagna S., Linden G. & Spik G. (1998), Structures of the O‑glycopeptides isolated from bovine milk component PP3. Glycoconjugate Journal, 15, 371-378.

Guimont C., Marchal E., Girardet J.M. & Linden G. (1997). Biological active factors in bovine milk and dairy by-products: influence on cell culture. Critical Reviews in Food Science and Nutrition, 37, 393-410.

Rudolf E., Girardet J.M., Bautz A.M. & Dournon C. (1997). Purification and partial characterization of peptidase-1, a sex-linked enzyme in Pleurodeles waltl (urodele amphibian). Biochemistry and Cell Biology, 75, 803-806.

Bernos E., Girardet J.M., Humbert G. & Linden G. (1997). Role of the O‑phosphoserine clusters in the interaction of the bovine milk alphas1‑, beta‑, kappa‑caseins and the PP3 component with immobilized iron(III) ions. Biochimica et Biophysica Acta (Protein Structure and Molecular Enzymology), 1337, 149‑159.

Girardet J.M. & Linden G. (1996). PP3 component of bovine milk: a phosphorylated whey glycoprotein. Journal of Dairy Research, 63, 333-350.

Girardet J.M., Coddeville B., Plancke Y., Strecker G., Campagna S. & Linden G. (1995). Structure of glycopeptides isolated from bovine milk component PP3. European Journal of Biochemistry (devenu FEBS Journal), 234, 939-946.

Le Roux Y., Girardet J.M., Humbert G., Laurent F. & Linden G. (1995). Proteolysis in samples of quarter milk with varying somatic cell counts. 2. component PP3 and beta-CN‑1P(f29‑105 and f29‑107) of the proteose-peptone fraction. Journal of Dairy Science, 78, 1298-1305.

Courthaudon J.L., Girardet J.M., Chapal C., Lorient D. & Linden G. (1995) Surface activity and competitive adsorption of milk component-3 and porcine pancreatic lipase at the dodecane-water interface. In Food Macromolecules and Colloids, pp. 58‑70 (Ed. E. Dickinson) London: Royal Society of Chemistry.

Etienne L., Girardet J.M. & Linden G. (1994). Growth promotion of Bifidobacterium animalis by bovine milk proteose-peptone. Le Lait, 74, 313‑323.

Girardet J.M., Saulnier F., Driou A., Linden G., Coddeville B. & Spik G. (1994). High performance electrophoresis chromatography of bovine milk component‑3 glycoproteins. Journal of Dairy Science, 77, 1205‑1216.

Mati A., Moulti-Mati F., Girardet J.M., Fokou E., Belleville-Nabet F., Nabet P. & Linden G. (1993). Mitogenic activity of hydrophobic fractions of proteose-peptone from cows’, ewes’ and goats’ milk measured with MARK 3 hybridoma culture. Journal of Dairy Research, 60, 443‑448.

Girardet J.M., Linden G., Loye S., Courthaudon J.L. & Lorient D. (1993). Study of mechanism of lipolysis inhibition by bovine milk proteose-peptone component‑3. Journal of Dairy Science, 76, 2156‑2163.

Mati A., Girardet J.M., Xenakis D. & Linden G. (1991). Isolement et caractérisation de la fraction hydrophobe des protéose-peptones des laits bovin, ovin et caprin. Le Lait, 71, 259‑273.

Saulnier F., Ferrero F., Choukri A., Girardet J.M. & Linden G. (1991). Estimation de la dénaturation des protéines par spectrofluorimétrie d’absorption moléculaire : applications aux lactosérums industriels. Le Lait, 71, 511‑518.

Girardet J.M., Mati A., Sanogo T., Etienne L. & Linden G. (1991). Fast protein liquid chromatography purification of hydrophobic fraction of bovine milk proteose-peptone and characterization by bidimensional electrophoresis. Journal of Dairy Research, 58, 85‑98.

Girardet J.M., Pâquet D. & Linden G. (1989). Effects of chromatographic parameters on the fractionation of whey proteins by anion exchange FPLC. Milchwissenschaft, 44, 692‑696.


Girardet J.M. (2014). Milk proteins as source of biofunctional peptides [Les protéines laitières sources de peptides biofonctionnels]. 1er Congrès International de Technologies Alimentaires et Contrôle Qualité des Aliments “AGROINNOV : Technologies Innovantes pour la Production Alimentaire”, Djerba, Tunisia, April, 25 – 27, 2014.

Girardet J.M. (2011). Antioxidant peptides from milk, unveiling and production by lactic acid bateria [Les peptides antioxydants du lait, leur mise en évidence et leur production par une bactérie lactique]. Antioxydants 2011 : Biodisponibilité, Métabolomique & Innovations Stratégiques. Société Française des Antioxydants (SFA), Paris, France, June, 09, 2011.