Rouhier N.

Career and scientific interests

I am a biochemist interested in basic plant science processes with a particular research focus on structure-function analysis of oxidoreductases and on redox post-translational modifications of proteins.

After a PhD in plant physiology, molecular biology and biochemistry, I have been recruited in 2004 as an assistant professor at the Université of Nancy, obtained an habilitation (HDR) in 2007 before gettting a professor position in 2011 at the newly created Université of Lorraine. For our research projects, we employ biochemical, biophysical, spectroscopic and structural biology approaches with Populus trichocarpa and Arabidopsis thaliana as model organisms to explore (i) the roles of the antioxidant/detoxification systems in the physiology of plants subject to environmental constraints and (ii) the maturation and roles of iron-sulfur proteins in plant organelles.

  • Redox post-translational modifications of proteins

In addition to performing structure-function analyses of thioredoxin (TRX) and glutaredoxin (GRX) family members which control most of the reversible oxidative modifications of protein cysteinyl residues, we are characterizing proteins of unknown function possessing one or several conserved CXXC motifs known to be particularly suited for disulfide bond formation but also other redox modifications. We hope to unravel new redox-regulated cellular processes or signaling pathways in poplar and more generally in plants which are controlled by redox reactions such as thiol-disulfide exchanges and understand how the functions of these proteins are controlled at the cellular level by TRX and GRX.

The same questions and strategies apply to sulfurtransferases, enzymes that catalyze the transfer of a sulfur atom from sulfur donors to nucleophilic sulfur acceptors (trans-persulfidation) by forming themselves persulfides. In this context, the intervention of the TRX and glutathione (GSH)/GRX reducing systems in the sulfur signaling/trafficking pathways is examined as both the TRX and GSH/GRX systems might modulate STR function and in particular be central to the formation of hydrogen sulfide, a molecule susceptible to trigger redox signalling cascades in several physiological situations.

Collaborators: C. Didierjean (Univ. Lorraine), JM. Herrmann (Kaiserslautern), S. Lemaire (CNRS – Sorbonne Université), A. Meyer (Bonn), B. Morgan (Saarbrücken), P. Rey (CEA Cadarache)

  • Molecular analysis of late steps of Fe-S cluster maturation in organelles

The general objective here is to understand the functioning of the Fe-S cluster assembly machineries in organelles and more precisely the molecular mechanisms controlling the second step, i.e. the trafficking of preformed Fe-S clusters from scaffold proteins (made in the first step) to final acceptors. After focusing for some time on the capacity of several plant GRXs to assemble [2Fe-2S] centers into homodimers or into heterodimers with BOLA proteins, we extended our research interest to the functional characterization of the associated protein families (NFUs, SUFA/ISCAs, IBA57s, HCF101/INDH) assumed to participate in the transfer of these Fe-S clusters together with GRX and BOLA. This functional analysis will combine plant genetics and physiology, molecular and structural biology and biochemistry approaches to determine whether these proteins assemble different types of Fe-S clusters and have specific interaction partners and what are the physiological and metabolic consequences of deleting these genes for plant development and physiology. Particular emphasis is given to the maturation of the photosynthetic and respiratory electron transfer complexes.

Collaborators: J. Balk (JIC, Norwich), MK. Johnson (Athens, USA), O. Keech (Umea), A. Meyer (Bonn), U. Mühlenhoff (Marburg), C. Remacle (Liège), F. Vignols (BPMP Montpellier)

  • Structure-Function analysis of glutathione transferases

Glutathione transferases (GSTs) constitute large multigenic families of more than 50 genes in terrestrial plants. They have three major biochemical functions. Those having a serinyl residue in their catalytic site (Ser-GSTs) possess GSH-conjugation activity. This catalytic activity is often described as required for xenobiotic detoxification. Those having a cysteinyl residue in their catalytic site (Cys-GSTs) possess the ability to catalyze the reverse reaction, i.e., the removal of glutathione from a number of structurally different heterocyclic molecules. The third function is the binding and/or transport of molecules/specialized metabolites without catalytic transformation, so-called ligandin function. It can be performed both by Ser-GSTs and Cys-GSTs. Although they are present in all kingdoms, the physiological functions of most plant GSTs remain elusive.

The current work is centered on the structure-function analysis of specific classes of glutathione transferases, focusing in particular on the isolation and identification of physiological substrates explaining their implication in biotic or abiotic stress responses. In particular, we are interested in GSTs regulated during poplar leaf infection by the rust fungus Melampsora larici-populina or regulated during iron starvation in A. thaliana.

The strategies used are to perform pull-down and affinity chromatographies, inhibition kinetics and thermal denaturation combining fluorescent probes and model ligands or plant extracts that are eventually separated by liquid ichromatography. We will not only focus on the previously characterized Cys-GSTs (lambda GSTs and glutathionyl hydroquinone reductases) but also on less explored or newly identified GST families. The nature (ligandin vs catalytic transformation) and the strength of the interactions will be validated by measuring binding constants using glutathionylated and non-glutathionylated molecules using biophycal approaches (ITC, Switchsense, fluorimetry). In parallel, the structure of GST alone or in complex with their ligand/substrate will be tentatively solved. Overall, we expect to decipher new intermediary steps of xenobiotic detoxification or of metabolic pathways requiring glutathione-conjugating or transport activities. New research questions have emerged recently from the observation that tau GST are able to bind porphyrins and hemes b in particular.

Collaborators: C. Didierjean (Univ. Lorraine), C. Dubos (BPMP, Montpellier), S. Lemaire (CNRS – Sorbonne Université)

 

Grants

-2019-2022 : Projet ANR-2018: MITOGLU: Dissecting the molecular interactions of mitochondrial glutaredoxin S15 in plants (coordinators N. Rouhier/A. Meyer)

-2018-2021 : Projet Lorraine Université Excellence, PEPS Mirabelle + : FeSprot: Maturation and structure-function analyses of iron-sulfur proteins (coordinator N. Rouhier)

– 2018-2021: Projet ANR 2017: MOBIFER: Dynamics of coumarin secretion by plant roots into the soil in the context of iron nutrition (coordinator C. Dubos)

– 2014-2017: Projet ANR 2013: Fe-S traffic: The cellular trafficking of iron-sulfur (Fe-S) clusters in plants (coordinator N. Rouhier)

– 2011-2013: Projet ANR 2010: FIRES: Functions of glutaredoxins in Iron and REdox sensing and Signaling in plants (coordinator N. Rouhier)

– 2009-2011: Projet INRA/FORMAS: The role of organellar glutaredoxins in iron-sulfur cluster biogenesis in plants: an interactomic study for the identification of protein partners (G. Wingsle (Umea Plant Science center, sweden)/ coordinator N. Rouhier)

– 2008-2010: Projet ANR Jeune Chercheur: The role of organellar CGFS containing glutaredoxins in iron-sulfur clusters assembly and biogenesis in plants (coordinator N. Rouhier)

– 2006-2008: Projet ANR, Réseau de Génomique végétale-Genoplante 2010: GNP05010P: Repair mechanisms of oxidized proteins in chloroplasts of Arabidopsis thaliana (coordinator Pascal Rey)

 

Scientific publications

2021

Przybyla-Toscano J, Boussardon C, Law S, Rouhier N, Keech O. Gene atlas of iron-containing proteins in Arabidopsis thaliana. Plant J. 2021 in press

Przybyla-Toscano J, Christ L, Keech O, Rouhier N. Iron-sulfur proteins in plant mitochondria: roles and maturation. J. Exp. Bot. 2021 in press

Rouhier N, Jacquot JP. News on the redox front – A follow-up of ABR volume 52: Oxidative stress and redox regulation in plants. Advances in Botanical Research. 2021 in press

Robe K, Conéjéro, G, Gao F, Lefebvre-Legendre L, Sylvestre-Gonon E, Rofidal V, Hem S, Rouhier N, Barberon M, Hecker A, Gaymard F, Izquierdo E, Dubos C. Coumarin accumulation and trafficking in Arabidopsis thaliana: a complex and dynamic process. New phytol. 2021, 229(4):2062-2079.

Berndt C, Christ L, Rouhier N, Mühlenhoff U. Glutaredoxins with Iron-Sulphur Clusters in Eukaryotes – Structure, Function and Impact on Disease. Biochim Biophys Acta-Bioenerg. 2021, 1862(1):148317.

2020

Azam T*, Przybyla-Toscano J*, Vignols F, Couturier J, Rouhier N, Johnson MK. [4Fe-4S] cluster trafficking mediated by Arabidopsis mitochondrial ISCA and NFU proteins. J Biol Chem. 2020, 295(52):18367-18378.

Berger N, Vignols F, Touraine B, Taupin-Broggini M, Rofidal V, Demolombe V, Santoni V, Rouhier N, Gaymard F, Dubos C. A global proteomic approach sheds new light on potential iron-sulfur client proteins of the chloroplastic maturation factor NFU3. Int J Mol Sci. 2020, 21(21):8121.

Azam T, Przybyla-Toscano J, Vignols F, Couturier J, Rouhier N, Johnson MK. The Arabidopsis Mitochondrial Glutaredoxin GRXS15 Provides [2Fe-2S] Clusters for ISCA-Mediated [4Fe-4S] Cluster Maturation. Int J Mol Sci. 2020, 21(23):9237.

Kammerscheit X, Hecker A, Rouhier N, Chauvat F, Cassier-Chauvat C. Methylglyoxal-detoxification revisited: role of a glutathione transferase in the model cyanobacterium Synechocystis PCC 6803. mBIO 2020, 11(4):e00882-20.

Roret T, Alloing G, Girardet JM, Perrot T, Dhalleine T, Couturier J, Frendo P, Didierjean C, Rouhier N. Sinorhizobium meliloti YrbA binds divalent metal cations using two conserved histidines. Biosci Rep. 2020 Sep 24:BSR20202956.

Valiauga B, Rouhier N, Jacquot JP, Čėnas N. Characterization of redox properties of FAD cofactor of Thermotoga maritima thioredoxin reductase. Chemija 2020.,31(3):191-195.

Sylvestre-Gonon E, Schwartz M, Girardet JM, Hecker A, Rouhier N. Is there a role for tau glutathione transferases in tetrapyrrole metabolism and retrograde signaling in plants? Phil. Trans. R. Soc. B, 2020, 375(1801):20190404.

Berger N, Vignols F, Przybyla-Toscano J, Roland M, Rofidal V, Touraine B, Zienkiewicz K, Couturier J, Feussner I, Santoni V, Rouhier N, Gaymard F, Dubos C. Identification of client iron-sulfur proteins of the chloroplastic NFU2 transfer protein in Arabidopsis thaliana. J Exp Bot. 2020, 71(14):4171-4187.

Moseler A, Selles B, Rouhier N, Couturier J. Novel insights into the diversity of the sulfurtransferase family in photosynthetic organisms with emphasis on oak. New phytol. 2020, 226(4):967-977.

*Roland M, Przybyla-Toscano J, Vignols F, Berger N, Azam T, Christ L, Santoni V, Wu HC, Dhalleine T, Johnson MK, Dubos C, Couturier J, Rouhier N. The plastidial Arabidopsis thaliana NFU1 protein binds and delivers [4Fe-4S] clusters to specific client proteins. J Biol Chem. 2020, 295(6):1727-1742.

*representative ‘Plant Biology’ article for in JBC the 2020 retrospective collection called “The year in JBC: 2020.”

2019

Selles B, Moseler A, Rouhier N, Couturier J. Rhodanese domain-containing sulfurtransferases: multifaceted proteins involved in sulfur trafficking in plants. J. Exp. Bot. 2019, 70(16):4139-4154.

Gurrieri L, Distefano L, Pirone C, Horrer D, Seung D, Zaffagnini M, Rouhier N, Trost P, Santelia D, Sparla F. The Thioredoxin-Regulated α-Amylase 3 of Arabidopsis thaliana Is a Target of S-Glutathionylation. Front Plant Sci. 2019, 10:993.

de Guillen K, Lorrain C, Tsan P, Barthe P, Petre B, Saveleva N, Rouhier N, Duplessis S, Padilla A, Hecker A. Structural genomics applied to the rust fungus Melampsora larici-populina reveals two candidate effector proteins adopting cystine knot and NTF2-like protein folds. Sci Rep. 2019, 9(1):18084

Touraine B, Vignols F, Przybyla-Toscano J, Ischebeck T Dhalleine T, Wu HC, Magno C, Berger N, Couturier J, Dubos C, Feussner I, Caffarri S, Havaux M, Rouhier N*, Gaymard F*. The iron-sulfur protein NFU2 plays a predominant role in branched-chain amino acid synthesis in Arabidopsis roots. J Exp Bot. 2019, 70(6):1875-1889

Zannini F, Moseler A, Bchini R, Dhalleine T, Meyer AJ, Rouhier N, Couturier J. The thioredoxin-mediated recycling of Arabidopsis thaliana GRXS16 relies on a conserved C-terminal cysteine. Biochim Biophys Acta-Gen Subj. 2019, 1863(2):426-436.

Meyer AJ, Riemer J, Rouhier N. Oxidative protein folding: state-of-the-art and current avenues of research in plants. New Phytol. 2019, 221(3):1230-1246.

Zaffagnini M, Fermani S, Marchand CH, Costa A, Sparla F, Rouhier N, Geigenberger P, Lemaire SD, Trost P. redox homeostasis in photosynthetic organisms : novel and established thiol-based molecular mechanisms. Antioxid Redox Signal. 2019, 31(3):155-210.

Sylvestre-Gonon E, Law S, Schwartz M, Robe K, Keech O, Didierjean C, Dubos C, Rouhier N* and Hecker A*. Functional, structural and biochemical features of plant serinyl-glutathione transferases. Front Plant Sci. 2019, 10:608.

Rey P, Taupin-Broggini M, Couturier J, Vignols F, Rouhier N. Is there a role for glutaredoxins and BOLAs in the perception of the cellular iron status in plants? Front Plant Sci. 2019, 10:712.

2018

Gao H, Azam T, Randeniya S, Couturier J, Rouhier N, Johnson MK. Characterization and Maturation of the Fe-S Center in Arabidopsis thaliana Dihydroxyacid Dehydratase. J Biol Chem 2018, 293(12):4422-4433.

Chibani K, Saul F, Didierjean C, Rouhier N, Haouz A. Structural snapshots along the reaction mechanism of the atypical poplar thioredoxin-like2.1. Febs Lett. 2018, 592(6):1030-1041.

Uzarska MA, Przybyla-Toscano J, Spangar F, Zannini F, Lill R, Mühlenhoff U, Rouhier N. Conserved functions of Arabidopsis mitochondrial late-acting maturation factors in the trafficking of iron‑sulfur clusters. Biochim Biophys Acta. Mol cell res. 2018, 1865(9):1250-1259.

Plomion C, Aury JM, Amselem J, Leroy T, Murat F, Duplessis S, Faye S, Francillonne N, Labadie K, Le Provost G, Lesur I, Bartholomé J, Faivre-Rampant P, Kohler A, Leplé JC, Chantret N, Chen J, Diévart A, Alaeitabar T, Barbe V, Belser C, Bergès H, Bodénès C, Bogeat-Triboulot MB, Bouffaud ML, Brachi B, Chancerel E, Cohen D, Couloux A, Da Silva C, Dossat C, Ehrenmann F, Gaspin C, Grima-Pettenati J, Guichoux E, Hecker A, Herrmann S, Hugueney P, Hummel I, Klopp C, Lalanne C, Lascoux M, Lasserre E, Lemainque A, Desprez-Loustau ML, Luyten I, Madoui MA, Mangenot S, Marchal C, Maumus F, Mercier J, Michotey C, Panaud O, Picault N, Rouhier N, Rué O, Rustenholz C, Salin F, Soler M, Tarkka M, Velt A, Zanne AE, Martin F, Wincker P, Quesneville H, Kremer A, Salse J. Oak genome reveals facets of long lifespan. Nat Plants. 2018, 4(7):440-452.

Zannini F, Roret T, Przybyla-Toscano J, Dhalleine T, Rouhier N, Couturier J. Mitochondrial Arabidopsis thaliana TRXo isoforms bind an iron-sulfur cluster and reduce NFU proteins in vitro. Antioxidants. 2018, 7(10). pii: E142.

Przybyla-Toscano J, Roland M, Gaymard F, Couturier J, Rouhier N. Roles and maturation of iron-sulfur proteins in plastids. J Biol Inorg Chem. 2018, 23(4):545-566.

2017

Peleh V, Zannini F, Backes S, Rouhier N*, Herrmann JM*. Erv1 of Arabidopsis thaliana can directly oxidize mitochondrial intermembrane space proteins in the absence of redox-active Mia40. BMC Biology. 2017 15(1):106

Przybyla-Toscano J, Roret T, Couturier J, Rouhier N. Roles of class II glutaredoxins in the maturation of Fe-S proteins. 2017 Metalloprotein Active Site Assembly in Encyclopedia of Inorganic and bioinorganic chemistry Wiley & Sons.

Zannini F, Couturier J, Keech O, Rouhier N. In vitro alkylation methods for assessing the protein redox state. Methods Mol. Biol. 2017, 1653:51-64.

Pégeot H, Mathiot S, Perrot T, Gense F, Hecker A, Didierjean C, Rouhier N. Structural plasticity among glutathione transferase Phi members: natural combination of catalytic residues confers dual biochemical activities. FEBS J. 2017, 284:2442-2463.

Rey P, Becuwe N, Tourrette S, Rouhier N. Involvement of Arabidopsis glutaredoxin S14 in the maintenance of chlorophyll content. Plant Cell Environ. 2017, 40:2319-2332.

Keech O, Gardeström P, Kleczkowski LA, Rouhier N. The redox control of photorespiration: from biochemical and physiological aspects to biotechnological considerations. Plant Cell Environ. 2017, 40(4):553-569.

Shaikhali J, Rouhier N, Hecker A, Brännström K, Wingsle G. Covalent and non-covalent associations mediate MED28 homo-oligomerization. Journal of Plant Biochemistry & Physiology. 2017, 5:189.

Selles B, Zannini F, Couturier J, Jacquot JP, Rouhier N. Atypical protein disulfide isomerases (PDI): Comparison of the molecular and catalytic properties of poplar PDI-A and PDI-M with PDI-L1A. PLoS One. 2017, 12:e0174753.

Castella C, Mirtziou I, Seassau A, Boscari A, Montrichard F, Papadopoulou K, Rouhier N, Puppo A, Brouquisse R. Post-translational modifications of Medicago truncatula glutathione peroxidase 1 induced by nitric oxide. Nitric Oxide. 2017, 68:125-136.

Werner Ribeiro C, Baldacci-Cresp F, Pierre O, Larousse M, Benyamina S, Lambert A, Hopkins J, Castella C, Cazareth J, Alloing G, Boncompagni E, Couturier J, Mergaert P, Gamas P, Rouhier N, Montrichard F, Frendo P. Regulation of Differentiation of Nitrogen-Fixing Bacteria by Microsymbiont-Targeting of Plant Thioredoxin s1. Current Biology. 2017, 27(2):250-256.

2016

Jacquot JP, Couturier J, Didierjean C, Gelhaye E, Morel-Rouhier M, Hecker A, Plomion C, Gütle D, Rouhier N. Structural and Functional Characterization of Tree Proteins Involved in Redox Regulation: A new Frontier in Forest Science. Annals of Forest sciences. 2016, 1-16.

Lallement PA, Roret T, Tsan P, Gualberto JM, Girardet JM, Didierjean C, Rouhier N, Hecker A. Insights into ascorbate regeneration in plants: investigating the redox and structural properties of dehydroascorbate reductases from Populus trichocarpa. Biochem J. 2016, 473(6):717-31.

Petre B, Hecker A, Germain H, Tsan P, Sklenar J, Pelletier G, Séguin A, Duplessis S, Rouhier N. The Poplar Rust-Induced Secreted Protein (RISP) Inhibits the Growth of the Leaf Rust Pathogen Melampsora larici-populina and Triggers Cell Culture Alkalinisation. Front Plant Sci. 2016, 7:97.

Cardi M, Zaffagnini M, De Lillo A, Castiglia D, Chibani K, Gualberto JM, Rouhier N, Jacquot JP, Esposito S. Plastidic P2 glucose-6P dehydrogenase from poplar is modulated by thioredoxin m-type: Distinct roles of cysteine residues in redox regulation and NADPH inhibition. Plant Sci. 2016, 252:257-266.

Chrobok D, Law SR, Brouwer B, Lindén P, Ziolkowska A, Liebsch D, Narsai R, Szal B, Moritz T, Rouhier N, Whelan J, Gardestrom P, Keech O. Dissecting the metabolic role of mitochondria during developmental leaf senescence. 2016, Plant Physiol. 172:2132-2153.

2015

Moseler A, Aller I, Wagner S, Nietzel T, Przybyla-Toscano J, Mühlenhoff U, Lill R, Berndt C, Rouhier N, Schwarzländer M, Meyer AJ. The mitochondrial monothiol glutaredoxin S15 is essential for iron-sulfur protein maturation in Arabidopsis thaliana. 2015 Proc Natl Acad Sci USA 2015, 112(44):13735-40.

Valiauga B, Rouhier N, Jacquot JP, Čėnas N. Quinone- and nitroreductase reactions of Thermotoga maritima thioredoxin reductase. Acta Biochima Polonica. 2015, 62(2):303-9.

Shaikhali J, Davoine C, Brännström K, Rouhier N, Bygdell J, Björklund S, Wingsle G. Biochemical and redox characterization of the Mediator complex and its associated transcription factor GeBPL, a GLABROUS1 enhancer binding protein. Biochem J. 2015, 468(3):385-400.

Matamoros MA, Saiz A, Peñuelas M, Bustos-Sanmamed P, Mulet JM, Barja MV, Rouhier N, Moore M, James EK, Dietz KJ, Becana M. Function of glutathione peroxidases in legume root nodules. J Exp Bot. 2015, 66(10):2979-90.

Knuesting J, Riondet C, Maria C, Kruse I, Bécuwe N, König N, Berndt C, Tourrette S, Guilleminot-Montoya J, Herrero E, Gaymard F, Balk J, Belli G, Scheibe R, Reichheld JP, Rouhier N, Rey P.. Glutaredoxin S17 and its partner NF-YC11/NC2α contribute to maintenance of the shoot apical meristem in Arabidopsis under long-day photoperiod. Plant Physiol. 2015, 167(4):1643-58.

Rouhier N, Cerveau D, Couturier J, Reichheld JP, Rey P. Involvement of thiol-based mechanisms in plant development. Biochim. Biophys. Acta. General Subject 2015, 1850(8):1479-1496.

Couturier J, Przybyla-Toscano J, Roret T, Didierjean C, Rouhier N. The roles of glutaredoxins integrating Fe-S clusters: sensing, transfer or repair functions. Biochim. Biophys. Acta. Mol cell res. 2015, 1853(6):1513-1527.

Lallement PA, Meux E, Gualberto JM, Dumarcay S, Favier F, Didierjean C, Saul F, Haouz A, Morel-Rouhier M, Gelhaye E, Rouhier N, Hecker A. Glutathionyl-hydroquinone reductases from poplar are plastidial proteins which deglutathionylate glutathione-conjugated reduced and oxidized quinones. Febs Lett. 2015, 589(1):37-44.

2014

Pégeot H, Koh CS, Petre B, Mathiot S, Duplessis S, Hecker A, Didierjean C, Rouhier N. The poplar phi class glutathione transferase: expression, activity and structure of GSTF1. Front Plant Sci. 2014, 23;5:712.

Lallement PA, Brouwer B, Keech O, Hecker A, Rouhier N. The still mysterious roles of cysteine-containing glutathione transferases in plants. Frontiers in pharmacology. 2014, 20;5:192.

Jacquot JP, Couturier J, Didierjean C, Gelhaye E, Morel-Rouhier M, Hecker A, Plomion C, Gütle D, Rouhier N. Structural and Functional Characterization of Tree Proteins Involved in Redox Regulation: A new Frontier in Forest Science. Annals of Forest sciences. 2014, 1-16.

Roret T, Tsan P, Couturier J, Zhang B, Johnson MK, Rouhier N, Didierjean C. Structural and spectroscopic insights into BolA-glutaredoxin complexes. J Biol Chem 2014, 289(35):24588-98.

Roret T, Pégeot H, Couturier J, Mulliert G, Rouhier N, Didierjean C. X-ray structures of Nfs2, the plastidial cysteine desulfurase from Arabidopsis thaliana. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2014, 70(Pt 9):1180-5.

Petre B, Hacquard S, Duplessis S, Rouhier N. Genome analysis of poplar LRR-RLP gene clusters reveals RISP, a defense-related gene coding a candidate endogenous peptide elicitor. Front Plant Sci. 2014, 5:111.

Lallement PA, Meux E, Gualberto JM, Prosper P, Didierjean C, Saul F, Haouz A, Rouhier N, Hecker A. Structural and enzymatic insights into Lambda glutathione transferases from Populus trichocarpa, monomeric enzymes constituting an early divergent class specific to terrestrial plants. Biochem J. 2014, 462(1):39-52.

Dhalleine T, Rouhier N, Couturier J. Putative roles of glutaredoxin-BolA holo-heterodimers in plants. Plant Signal Behav. 2014, 9(3).

Wang L, Li Y, Jacquot JP, Rouhier N, Xia B. Characterization of poplar GrxS14 in different structural forms. Protein Cell 2014, 5(5):329-33.

Couturier J, Wu HC, Dhalleine T, Pégeot H, Sudre D, Gualberto JM, Jacquot JP, Gaymard F, Vignols F, Rouhier N. Monothiol glutaredoxin-BolA interactions: redox control of Arabidopsis thaliana BolA2 and SufE1. Mol Plant 2014, 7(1):187-205.

Cejudo FJ, Meyer AJ, Reichheld JP, Rouhier N, Traverso JA. Thiol-based redox homeostasis and signaling. Front. Plant Sci 2014, 5:266.

2013

Aller I, Rouhier N, Meyer A. Development of roGFP2-derived redox probes for measurement of the glutathione redox potential in the cytosol of severely glutathione deficient rml1 seedlings. Front Plant Sci. 2013, 16;4:506.

Couturier J, Jacquot JP, Rouhier N. Towards a refined classification of class I dithiol glutaredoxins from poplar: biochemical basis for the definition of two subclasses. Front Plant Sci. 2013, 18;4:518.

Cardi M, Chibani K, Castiglia D, Cafasso D, Pizzo E, Rouhier N, Jacquot JP, Esposito S. Overexpression, purification and enzymatic characterization of a recombinant plastidial glucose-6-phosphate dehydrogenase from barley (Hordeum vulgare cv. Nure) roots. Plant Physiol Biochem, 2013, 17;73C:266-273.

Zhang B, Bandyopadhyay S, Shakamuri P, Naik SG, Huynh BH, Couturier J, Rouhier N, Johnson MK. Monothiol Glutaredoxins Can Bind Linear [Fe3S4]+ and [Fe4S4]2+ Clusters in Addition to [Fe2S2]2+ Clusters: Spectroscopic Characterization and Functional Implications. J Am Chem Soc. 2013, 135(40):15153-64.

Gao H, Subramanian S, Couturier J, Naik SG, Kim SK, Leustek T, Knaff DB, Wu HC, Vignols F, Huynh BH, Rouhier N, Johnson MK. Arabidopsis thaliana Nfu2 Accommodates [2Fe-2S] or [4Fe-4S] Clusters and Is Competent for in Vitro Maturation of Chloroplast [2Fe-2S] and [4Fe-4S] Cluster-Containing Proteins. Biochemistry. 2013, 52(38):6633-45.

Couturier J, Touraine B, Briat JF, Gaymard F, Rouhier N. The iron-sulfur cluster assembly machineries in plants: current knowledge and open questions. Front Plant Sci. 2013, 24;4:259.

Zaffagnini M, Morisse S, Bedhomme M, Marchand CH, Festa M, Rouhier N, Lemaire SD, Trost P. Mechanisms of nitrosylation and denitrosylation of cytoplasmic glyceraldehyde-3-phosphate dehydrogenase from Arabidopsis thaliana. J Biol Chem. 2013, 288(31):22777-89.

Couturier J, Chibani K, Jacquot JP, Rouhier N. Cysteine-based redox regulation and signalling in plants. Front Plant Sci. 2013, 29;4:105.

Jacquot JP, Dietz KJ, Rouhier N, Meux E, Lallement PA, Selles B, and Hecker A. Redox regulation in plants: Glutathione and “Redoxin” related families. Springer Book Oxidative stress and redox regulation 2013

Messens J, Rouhier N, Collet JF. Redox Homeostasis. Springer Book Oxidative stress and redox regulation 2013

Benyamina SM, Baldacci-Cresp F, Couturier J, Chibani K, Hopkins J, Bekki A, de Lajudie P, Rouhier N, Jacquot JP, Alloing G, Puppo A, Frendo P. Two Sinorhizobium meliloti glutaredoxins regulate iron metabolism and symbiotic bacteroid differentiation. Env. Microbiol. 2013, 15(3):795-810

Mapolelo DT, Zhang B, Randeniya S, Albetel AN, Li H, Outten CE, Couturier J, Rouhier N, Johnson MK. Monothiol glutaredoxins and A-type proteins: Partners in Fe-S cluster trafficking. Dalt trans. 2013 42(9):3107-15.

Couturier J, Prosper P, Winger AM, Hecker A, Hirasawa M, Knaff DB, Gans P, Jacquot JP, Navaza A, Haouz A, Rouhier N. In the absence of thioredoxins, what are the reductants for peroxiredoxins in Thermotoga maritima? Antioxid Redox Signal. 2013, 18(13):1613-22

 

2012

Zaffagnini M, Bedhomme M, Marchand CH, Couturier J, Gao XH, Rouhier N, Trost P, Lemaire SD. Glutaredoxin S12: unique properties for redox signaling. Antioxid Redox Signal. 2012, 16:17-32.

Tarrago L, Kieffer-Jaquinod S, Lamant T, Marcellin MN, Garin JR, Rouhier N, Rey P. Affinity chromatography: a valuable strategy to isolate substrates of methionine sulfoxide reductases? Antioxid Redox Signal. 2012, 16:79-84.

Selles B, Hugo M, Trujillo M, Srivastava V, Wingsle G, Jacquot JP, Radi R, Rouhier N. Hydroperoxide and peroxynitrite reductase activity of poplar thioredoxin-dependent glutathione peroxidase 5 : kinetics, catalytic mechanism and oxidative inactivation. Biochem J. 2012, 442(2):369-80.

Chibani K, Tarrago L, Gualberto JM, Wingsle G, Rey P, Jacquot JP, Rouhier N. Atypical thioredoxins in poplar: the glutathione-dependent thioredoxin-like 2.1 supports the activity of target enzymes possessing a single redox active cysteine. Plant Physiol. 2012, 159(2):592-605.

Bedhomme M, Adamo M, Marchand CH, Couturier J, Rouhier N, Lemaire SD, Zaffagnini M, Trost P. Glutathionylation of cytosolic glyceraldehyde-3-phosphate dehydrogenase from the model plant Arabidopsis thaliana is reversed by both glutaredoxins and thioredoxins in vitro. Biochem J. 2012, 445(3):337-47.

Petre B, Morin E, Tisserant E, Hacquard S, Da Silva C, Poulain J, Delaruelle C, Rouhier N, Martin F, Kohler A, Duplessis S. RNA-Seq of early-infected poplar leaves by the rust pathogen Melampsora laricipopulina uncovers PtSultr3;5, a fungal-induced host sulfate transporter. Plos one, 2012;7(8):e44408.

Wang L, Ouyang B, Li Y, Feng Y, Jacquot JP, Rouhier N, Xia B. Glutathione regulates the transfer of iron-sulfur cluster from monothiol and dithiol glutaredoxins to apo ferredoxin. Protein Cell, 2012, 3(9):714-21.

Anusevičius T, Misevičiene L, Šarlauskas J, Rouhier N, Jacquot JP, Čėnas N. Quinone- and nitroreductase reactions of Thermotoga maritime peroxiredoxin-nitroreductase hybrid enzyme. Arch Biochem Biophys, 2012, 528(1):50-6.

Couturier J, Vignols F, Jacquot JP, Rouhier N. Glutathione- and glutaredoxin-dependent reduction of methionine sulfoxide reductase A. Febs letters, 2012, 586(21):3894-9.

 

2011

Wang L, Ren X, Li Y, Rouhier N, Jacquot JP, Jin C, Xia B. (1)H, (13)C, and (15)N resonance assignments of reduced GrxS14 from Populus tremula × tremuloides. Biomol NMR Assign. 2011 5(1):121-4.

Selles B, Jacquot JP, Rouhier N. Comparative genomic study of protein disulfide isomerases from photosynthetic organisms. Genomics. 2011, 97(1):37-50.

Bunet R, Song L, Mendes MV, Corre C, Hotel L, Rouhier N, Framboisier X, Leblond P, Challis GL and Aigle B. Characterization and manipulation of the pathway-specific late regulator AlpW reveals Streptomyces ambofaciens as a new producer of kinamycins. J Bacteriol. 2011, 193(5):1142-53

Petre B, Major I, Rouhier N, Duplessis S. Genome-wide analysis of eukaryote thaumatin-like proteins (TLPs) with an emphasis on poplar. BMC Plant Biol. 2011, 15:11:33.

Cardi M, Chibani K, Cafasso D, Rouhier N, Jacquot JP, Esposito S. Abscisic acid effects on activity and expression of barley (Hordeum vulgare) plastidial glucose-6-phosphate dehydrogenases. J Exp Bot. 2011, 62:4013-23.

Duplessis S, Cuomo CA, Lin YC, Aerts A, Tisserant E, Veneault-Fourrey C, Joly DL, Hacquard S, Amselem J, Cantarel BL, Chiu R, Couthinho P, Feau N, Field M, Frey P, Gelhaye E, Goldberg J, Grabherr M, Kodira CD, Kohler A, Kües U, Lindquist EA, Lucas S, Mago R, Mauceli E, Morin E, Murat C, Pangilinan JL, Park R, Pearson M, Quesneville H, Rouhier N, Sakthikumar S, Salamov AA, Schmutz J, Selles B, Shapiro H, Tangay P, Tuskan GA, Henrissat B, Van de Peer Y, Rouzé P, Ellis JG, Dodds PN, Schein JE, Zhong S, Hamelin RC, Grigoriev IV, Szabon LJ, Martin F. Obligate Biotrophy Features Unraveled by the Genomic Analysis of Rust Fungi. Proc Natl Acad Sci USA 2011, 108(22):9166-71

Chibani K, Tarrago L, Schürmann P, Jacquot JP, Rouhier N. Biochemical properties of poplar thioredoxin z. FEBS Lett. 2011, 585:1077-81.

Méndez A, Matamoros MA, Bustos-Sanmamed P, Dietz KJ, Cejudo FJ, Rouhier N, Sato S, Tabata S, Becana M. Peroxiredoxins and NADPH-Dependent Thioredoxin Systems in the Model Legume Lotus japonicus. Plant Physiol. 2011, 156:1535-47.

Couturier J, Stroher E, Albetel AN, Roret T, Muthuramalingam M, Tarrago L, Seidel T, Tsan P, Jacquot JP, Johnson MK, Dietz KJ, Didierjean C, Rouhier N. Arabidopsis chloroplastic glutaredoxin C5 as a model to explore the molecular determinants for iron-sulfur cluster binding into glutaredoxins. J Biol Chem. 2011, 286:27515-27.

Rouhier N, Jacquot JP, Misevčiene L, Anusevičius Z, Šarlauskas J, Čėnas N. Two-electron reduction of nitroaromatic compounds by Thermotoga maritima hybrid peroxiredoxin-nitroreductase enzyme. Chemija 2011, 22 :143-148.

Hacquard S, Petre B, Frey P, Hecker A, Rouhier N, Duplessis S. The poplar-poplar rust interaction: insights from genomics and transcriptomics. J. Pathogens 2011;2011:716041.

 

2010

Couturier, J., Didierjean, C., Jacquot, J.-P., Rouhier, N. Engineered mutated glutaredoxins mimicking peculiar plant class III glutaredoxins bind iron-sulfur centers and possess reductase activity. Biochem Biophys Res Comm 2010, 403(3-4):435-41.

Rouhier N, Couturier J, Johnson MK, Jacquot JP. Glutaredoxins: roles in iron homeostasis. Trends Biochem Sci. 2010, 35(1):43-52.

Chibani K, Couturier J, Selles B, Jacquot JP, Rouhier N. The chloroplastic thiol reducing systems: dual functions in the regulation of carbohydrate metabolism and regeneration of antioxidant enzymes, emphasis on the poplar redoxin equipment. Photosynth Res. 2010, 104(1):75-99.

Rouhier N. Plant glutaredoxins: pivotal players in redox biology and iron-sulphur centre assembly. New Phytol 2010, 186(2):365-72.

Jacquot JP, Rouhier N. Introduction. Photosynth Res. 2010, 104(1):1-3.

 

2009

Ramos J, Matamoros M, Nayal L, James EK, Rouhier N, Sato S, Tabata S, Becana M. The glutathione peroxidase gene family of Lotus japonicus. Characterization of genomic clones, expression analyses and immunolocalization in legumes. New Phytol. 2009, 181(1):103-14.

Chibani K, Wingsle G, Jacquot JP, Gelhaye E, Rouhier N. Comparative genomic study of the thioredoxin family in photosynthetic organisms with emphasis on Populus trichocarpa. Mol. Plant. 2009, 2 :308-322

Couturier J, Koh CS, Zaffagnini M, Winger A, Gualberto JM, Corbier C, Decottignies P, Jacquot JP, Lemaire SD, Didierjean C, Rouhier N. Structure function relationship of the chloroplastic GrxS12 with an atypical WCSYS active site. J Biol Chem. 2009, 284(14):9299-310.

Srivastava V, Kumar Srivastava M, Chibani K, Nilsson R,Rouhier N, Melzer M, Wingsle G. Alternative Splicing Studies of the ROS Gene Network in Populus Reveal Two Isoforms of High Iso-electric Point SuperoxideDismutase. Plant physiol. 2009, 149(4):1848-59.

Tarrago L, Laugier E, Zaffagnini M, Marchand C, Le Maréchal P, Rouhier N, Lemaire SD, Rey P. Regeneration mechanisms of Arabidopsis thaliana methionine sulfoxide reductases B by glutaredoxins and thioredoxins.J Biol Chem. 2009, 284(28):18963-71.

Couturier J, Jacquot JP, Rouhier N. Evolution and diversity of glutaredoxins in photosynthetic organisms. Cell Mol Life Sci. 2009, 66(15):2539-57.

Jacquot JP, Eklund H, Rouhier N, Schürmann P. Structural and evolutionary aspects of thioredoxin reductases in photosynthetic organisms. Trends Plant Sci. 2009, 14(6):336-43.

Selles B, Rouhier N, Chibani K, Couturier J, Gama F, Jacquot JP. Chapter 13 Glutaredoxin. The Missing Link Between Thiol-Disulfide Oxidoreductases and Iron Sulfur Enzymes. 2009 Advances in Botanical Research 52 (C), pp. 405-436

 

2008

Barbey C, Rouhier N, Haouz A, Navaza A, Jacquot JP. Expression, purification, crystallization and preliminary X-ray analysis of the peroxiredoxin domain of a larger natural hybrid protein from Thermotoga maritima. Acta Crystallogr Sect F Struct Biol Cryst Commun.  2008, 64:29-31.

Martin F, Aerts A, Ahrén D, Brun A, Danchin EG, Duchaussoy F, Gibon J, Kohler A, Lindquist E, Pereda V, Salamov A, Shapiro HJ, Wuyts J, Blaudez D, Buée M, Brokstein P, Canbäck B, Cohen D, Courty PE, Coutinho PM, Delaruelle C, Detter JC, Deveau A, DiFazio S, Duplessis S, Fraissinet-Tachet L, Lucic E, Frey-Klett P, Fourrey C, Feussner I, Gay G, Grimwood J, Hoegger PJ, Jain P, Kilaru S, Labbé J, Lin YC, Legué V, Le Tacon F, Marmeisse R, Melayah D, Montanini B, Muratet M, Nehls U, Niculita-Hirzel H, Oudot-Le Secq MP, Peter M, Quesneville H, Rajashekar B, Reich M, Rouhier N, Schmutz J, Yin T, Chalot M, Henrissat B, Kües U, Lucas S, Van de Peer Y, Podila GK, Polle A, Pukkila PJ, Richardson PM, Rouzé P, Sanders IR, Stajich JE, Tunlid A, Tuskan G, Grigoriev IV. The genome of Laccaria bicolor provides insights into mycorrhizal symbiosis. Nature. 2008, 452:88-92.

Bunet R, MendesMV, RouhierN, PangX, HotelL, DecarisB, LeblondP, AigleB. Regulation of the angucyclinone antibiotic alpomycin in Streptomyces ambofaciens by the autoregulator receptor AlpZ and its specific ligand. J. Bacteriol. 2008, 190:3293-305.

Gama F, Bréhelin C, Gelhaye E, Meyer Y, Jacquot JP, Rey P, Rouhier N. Biochemical and functional analysis of chloroplastic Prx IIE. Physiol Plant. 2008, 133:599-610.

Bandyopadhyay S, Gama F, Molina-Navarro MM, Gualberto JM, Claxton R, Naik SG, Huynh BH, Herrero E, Jacquot JP, Johnson MK, Rouhier N. Chloroplast monothiol glutaredoxins as scaffold proteins for the assembly and delivery of [2Fe-2S] clusters. EMBO J. 2008, 27:1122-33

Morel M, Kohler A, Martin F, Gelhaye E, Rouhier N. Comparison of the thiol-dependent antioxidant systems in the ectomycorrhizal Laccaria bicolor and the saprotrophic Phanerochaete chrysosporium. New Phytol. 2008, 180(2):391-407.

Koh CS, Navrot N, Didierjean C, Rouhier N, Hirasawa M, Knaff DB, Wingsle G, Samian R, Jacquot JP, Corbier C, Gelhaye E. An atypical catalytic mechanism involving three cysteines of thioredoxin. J Biol Chem. 2008, 283:23062-23072.

Rouhier N, Jacquot JP. Getting sick may help plants overcome abiotic stress. New Phytol. 2008, 180 : 738-741.

Rouhier N, Lemaire SD, Jacquot JP. The role of glutathione in photosynthetic organisms : emerging functions for glutaredoxin and glutathionylation. Annu. Rev. Plant Biol. 2008, 59:143-166.

Rouhier N, Koh CS, Gelhaye E, Corbier C, Tete-Favier F, Didierjean C, Jacquot JP. Redox based anti-oxidant systems in plants: biochemical and structural analyses. Biochim. Biophys. Acta. 2008, 1780:1249-60.

 

2007

Gama F, Keech O, Eymery F, Finkemeier I, Gelhaye E, Gardeström P, Dietz KJ, Rey P, Jacquot JP, Rouhier N. The mitochondrial type II peroxiredoxin from poplar Physiol. Plant. 2007, 129(1) :196-206.

Rouhier N, Kauffmann B, Tete-Favier F, Palladino P, Gans P, Branlant G, Jacquot JP, Boschi-Muller S. Functional and structural aspects of poplar cytosolic and plastidial type a methionine sulfoxide reductases. J. Biol. Chem. 2007, 282(5):3367-78.

Rouhier N, Hideaki Unno H, BandyopadhyayS, Masip L,Kim SK, Hirasawa M, Gualberto JM, Lattard V, Kusunoki M, Knaff DB, Georgiou G, Hase T, Johnson MK, Jacquot JP. Functional, structural and spectroscopic characterization of a glutathione-ligated [2Fe-2S] cluster in poplar glutaredoxin C1. Proc Natl Acad Sci USA 2007, 104(18):7379-84.

Koh CS, Didierjean C, Navrot N,  Panjikar S, Mulliert G, Rouhier N, Jacquot JP, Aubry A, Shawkataly O, Corbier C. Crystal structure of a poplar thioredoxin peroixdase which exhibits the structure of glutathione peroxidases : insights into redox driven conformational changes. J. Mol. Biol. 2007, 370(3):512-29.

Passardi F, Zamocky M, Cosio C,  Rouhier N, Theiler G, Penel C, and Dunand C. PeroxiBase: the peroxidase database. Phytochemistry 2007, 68(12):1605-11.

Meyer AJ, Brach T, Marty L, Kreye S, Rouhier N, Jacquot JP, Hell R. Redox-sensitive GFP in Arabidopsis thaliana is a quantitative biosensor for the redox potential of the cellular glutathione redox buffer. Plant J. 2007, 52(5):973-86

Vieira Dos Santos C, Laugier E, Tarrago L, Massot V, Issakidis-Bourguet E, Rouhier N Rey P.Specificity of thioredoxins and glutaredoxins as electron donors to two distinct classes of Arabidopsis plastidial methionine sulfoxide reductases B FEBS Lett. 2007, 581(23):4371-6.

Navrot N, Rouhier N, Gelhaye E, Jacquot JP. ROS generation and antioxidant systems in plant mitochondria. Physiol. Plantarum 2007, 129(1):185-195.

2006

Rouhier N, Gama F, Wingsle G, Gelhaye E, Gans P, Jacquot JP. Engineering artificial functional hybrid proteins between poplar peroxiredoxin II and glutaredoxin or thioredoxin. Biochem. Biophys. Res Comm. 2006, 341(4):1300-1308.

Feng Y,  Zhong N, Rouhier N, Hase T, Kusunoki K, Jacquot JP, Jin C, Xia B. Structural insight into poplar glutaredoxin C1 with a bridging iron sulfur cluster at the active site. Biochemistry. 2006, 45(26):7998-8008

Noguera-Mazon V, Lemoine J, Walker O, Rouhier N, Salvador A, Jacquot JP, Lancelin JM, Krimm I. Glutathionylation induces the dissociation of 1-Cys D-peroxiredoxin non-covalent homodimer. J Biol Chem. 2006, 281(42):31736-42.

Gelhaye E, Navrot N, Macdonald IK, Rouhier N, Lloyd Raven E, Jacquot JP. Ascorbate Peroxidase-Thioredoxin Interaction. Photosynth Res. 2006, 89(3):1-8.

Navrot N, Collin V, Gualberto J, Gelhaye E, Hirasawa M, Rey P, Knaff DB, Issakidis E, Jacquot JP, Rouhier N. Plant glutathione peroxidases are functional peroxiredoxins distributed in several subcellular compartments and regulated during biotic and abiotic stresses. Plant Physiol. 2006, 142(4):1364-79.

Rouhier N, Couturier J, Jacquot JP. Genome-wide analysis of plant glutaredoxin systems. J. Exp. Bot. 2006, 57(8):1685-96.

Navrot N, Gelhaye E, Jacquot JP, Rouhier N. Identification of a new family of plant proteins loosely related to glutaredoxins with four CxxC motives. Photosynth. Res. 2006. 89(2):71-9.

Rouhier N, Vieira Dos Santos C, Tarrago L Rey P. Plant methionine sulfoxide reductase A and B multigenic families. Photosynth Res. 2006, 89(3):1-16.

2005

Rey P, Cuiné S, Eymery F, Garin J, Court M, Jacquot JP, Rouhier N, Broin M. Analysis of the proteins targeted by CDSP32, a plastidic thioredoxin participating in oxidative stress responses. Plant J, 2005, 41:31-42.

Echalier A, Trivelli X, Corbier C, Rouhier N, Walker O, Tsan P,  Jacquot JP, Krimm I, Lancelin JM. X-ray and NMR studies of a homodimeric D (Type II) peroxiredoxin reduced by both glutaredoxin and thioredoxin : Prx-Prx interfaces in the Prx family. Biochemistry, 2005, 44:1755-67.

Mabicka A, Dumarcay S, Rouhier N, Linder M, Jacquot JP, Gerardin P, Gelhaye E. Synergistic wood preservatives involving EDTA, inorganic 1076 and 2-hydroxypyridine-N-oxide. International biodeterioration and biodegradation. 2005, 55 (3), 203-211.

Feng Y, Rouhier N, Jacquot JP, Xia B. Letter to the Editor: (1)H, (15)N, and (13)C resonance assignments of reduced glutaredoxin C1 from Populus tremula x tremuloides. J Biomol NMR. 2005, 31:263-4.

Vieira Dos Santos C, Cuine S, Rouhier N, Rey P. The Arabidopsis plastidic methionine sulfoxide reductase B proteins. Sequence and activity characteristics, comparison of the expression with plastidic methionine sulfoxide reductase a, and induction by photooxidative stress. Plant Physiol. 2005, 138(2):909-22.

Rouhier N, Villarejo A, Srivastava M, Gelhaye E, Keech O, Droux M, Finkemeier I, Samuelsson G, Dietz KJ, Jacquot JP, WingsleG. Identification of plant glutaredoxin targets. Antiox Redox Signal. 2005, 7(7-8):919-929.

Noguera V, Walker O, Rouhier N, Jacquot JP, Krimm I, Lancelin JM. NMR reveals a novel glutaredoxin glutaredoxin interaction interface. J. Mol. Biol. 2005, 353(3):629-41.

Gelhaye E, RouhierN, Navrot N, Jacquot JP. The plant thioredoxin systems. Cell. Mol. Life Sci. 2005, 62:24-35.

Rouhier N, Jacquot JP. The plant multigenic family of thiol peroxidases. Free Radic. Biol. Med. 2005, 38 (11):1413-21.

2004

Rouhier N, Gelhaye E, Corbier C Jacquot JP. Active site mutagenesis and phospholipid hydroperoxide reductase activity of poplar type II peroxiredoxin. Physiol. Plant. 2004, 120(1):57-62.

Gelhaye E, Rouhier N, Jacquot JP. Evidence for a subgroup of thioredoxin h that requires GSH/Grx for its reduction. FEBS Lett. 2004, 555(3): 443-448.

Rouhier N, Gelhaye E, Gualberto J, Jordy MN, De Fay E, Hirasawa M, Duplessis S, Lemaire S, Frey P, Martin F, Manieri W, Knaff DB, Jacquot JP. Poplar peroxiredoxin Q : a thioredoxin-linked chloroplast antioxidant functional in pathogen defense. Plant Physiol. 2004, 134(3):1027-38.

Balmer Y, Vensel WH, Tanaka CK, Hurkman WJ, Gelhaye E, RouhierN, JacquotJP, Manieri W, Schürmann P, Droux M, Buchanan BB. Thioredoxin links redox to the regulation of fundamental processes of plant mitochondria. Proc. Natl. Acad. Sci. USA 2004, 101(8):2642-7.

Jacquot JP, RouhierN, Navrot N, Decottignies JP. Generation of polyclonal antibodies against multiple proteins in a single rabbit and subsequent isolation of the specific immunoglobulins as a tool for proteomics research. Biotechnol. App. Bioc. 2004, 40(Pt 2):181-5.

Bouillac S, Rouhier N, Tsan P, Jacquot JP, Lancelin JM. 1H, (13)C and (15)N NMR assignment of the homodimeric poplar phloem type II peroxiredoxin. J Biomol NMR. 2004 Sep;30(1):105-6.

Noguera V, Rouhier N, Krimm I, Jacquot JP, Lancelin JM. 1H, 13C and 15N NMR assigment of the homodimeric poplar phloem peroxiredoxin. J Biomol NMR, 2004 Oct;30(2):219-20.

Gelhaye E, Rouhier N, Gerard J, Jolivet Y, Gualberto J, Navrot N, Ohlsson PI, Wingsle G, Hirasawa M, Knaff DB, Wang H, Dizengremel P, Meyer Y, Jacquot JP. A specific form of thioredoxin h occurs in plant mitochondria and regulates the alternative oxidase. Proc Natl Acad Sci USA 2004, 101(40):14545-50.

Rouhier N, Gelhaye E, Jacquot JP. Plant glutaredoxins : still mysterious reducing systems. Cell. Mol. Life Sci. 2004, 61:1266-77.

Gelhaye E, Rouhier N, Jacquot JP. The thioredoxin h system of higher plants. Plant Physiol. Biochem. 2004, 42(4):265-71.

2003

Rouhier N, Vlamis-Gardikas A, Lillig CH, Berndt C, Schwenn JD, Holmgren A, Jacquot JP. Characterization of the redox properties of poplar glutaredoxin. Antioxid. Redox Signal. 2003, 5(1):15-22.

Gelhaye E, Rouhier N, Vlamis-Gardikas A, Girardet JM, Sautière PE, Sauzet F, Martin F, Jacquot JP. Identification and characterization of a third thioredoxin h in poplar. Plant Physiol. Bioch. 2003, 41 : 629-635.

D’Ambrosio K, Kauffmann B, Rouhier N, Benedetti E, Jacquot JP, Aubry A, Corbier C. Crystallization and preliminary X-ray studies of the glutaredoxin from poplar in complex with glutathione. Acta Crystallogr. D Biol. Crystallogr. 2003, 59(Pt 6):1043-1045.

Rouhier N, Jacquot JP. Molecular and catalytic properties of a peroxiredoxin-glutaredoxin hybrid from Neisseria meningitidis. FEBS Lett. 2003, 554(1-2):149-53.

2002

Rouhier N, Gelhaye E, Jacquot JP. Exploring the active site of plant glutaredoxin by site-directed mutagenesis. FEBS Lett. 2002, 511(1-3):145-9.

Rouhier N, Gelhaye E, Jacquot JP. Glutaredoxin-dependent peroxiredoxin from poplar: protein-protein interaction and catalytic mechanism. J. Biol. Chem. 2002, 277(16):13609-14.

Rouhier N, Gelhaye E, Sautiere PE, Jacquot JP. Enhancement of poplar glutaredoxin expression by optimization of the cDNA sequence. Prot. Expr. Purif. 2002, 24(2):234-41.

Gelhaye E, Rouhier N, Laurent P, Sautiere PE, Martin F, Jacquot JP. Isolation and characterization of an extended thioredoxin h from poplar.Physiol. Plant. 2002, 114(2):165-171.

Echalier A, Corbier C, Rouhier N, Jacquot JP, Aubry A. Crystallization and preliminary X-ray data of a bifunctional peroxiredoxin from poplar. Acta Crystallogr. D Biol. Crystallogr. 2002, 58(Pt 9):1501-3.

Rouhier N, Jacquot JP. Plant peroxiredoxins: alternative hydroperoxide scavenging enzymes. Photosynth. Res. 2002, 74: 259-268.

Jacquot JP, Gelhaye E, Rouhier N, Corbier C, Didierjean C, Aubry A. Thioredoxins and related proteins in photosynthetic organisms: molecular basis for thiol dependent regulation. Biochem. Pharmacol. 2002, 64(5-6):1065-9.

Jacquot JP, Rouhier N, Gelhaye E. Redox control by dithiol-disulfide exchange in plants: I. The chloroplastic systems. Ann. N Y Acad. Sci. 2002, 973:508-19.

Rouhier N, Gelhaye E, Jacquot JP. Redox control by dithiol-disulfide exchange in plants: II. The cytosolic and mitochondrial systems. Ann. N Y Acad. Sci. 2002, 973:520-8.

2001

 Rouhier N, Gelhaye E, Sautiere PE, Brun A, Laurent P, Tagu D, Gerard J, de Fay E, Meyer Y, Jacquot JP. Isolation and characterization of a new peroxiredoxin from poplar sieve tubes that uses either glutaredoxin or thioredoxin as a proton donor. Plant Physiol. 2001, 127(3):1299-309