Tuber melanosporum DB

You are here: MycorWeb Genome resources Tuber melanosporum DB EST & Gene Sequences Gene card

Gene Name: GSTUMT00010022001
Length(AA) : 669
Localization : scaffold_65
Number of EST contigs matching the gene: 2
Number of ESTs : 5
  User annotation : No user annotation

Blast Results:

Nr Definition: hypothetical protein AN0236.2
Specie: [Aspergillus nidulans FGSC A4]
Accession: ref|XP_657840.1| Score: 270 E_value: 1e-21
KEGG Definition: hypothetical protein
Accession: ani:AN0236.2 Score: 270 E_value: 7e-22
KOG Definition: Splicing factor 1/branch point binding protein (RRM superfamily)
Classification: [RNA processing and modification].
Kog Number: KOG0119 Score: 244 E_value: 3e-21
PFAM Definition: Atrophin-1, Atrophin-1 family. Atrophin-1 is the protein product of the dentatorubral-pallidoluysian atrophy (DRPLA) gene. DRPLA OMIM:125370 is a progressive neurodegenerative disorder. It is caused by the expansion of a CAG repeat in the DRPLA gene on chromosome 12p. This results in an extended polyglutamine region in atrophin-1, that is thought to confer toxicity to the protein, possibly through altering its interactions with other proteins. The expansion of a CAG repeat is also the underlying defect in six other neurodegenerative disorders, including Huntington's disease. One interaction of expanded polyglutamine repeats that is thought to be pathogenic is that with the short glutamine repeat in the transcriptional coactivator CREB binding protein, CBP. This interaction draws CBP away from its usual nuclear location to the expanded polyglutamine repeat protein aggregates that are characteristic of the polyglutamine neurodegenerative disorders. This interferes with CBP-mediated transcription and causes cytotoxicity..
Pfam Number: pfam03154 Score: 279 E_value: 3e-25
Swissprot: No Hit Found

SignalP

SignalP 3.0 predicts the presence and location of signal peptide cleavage sites in amino acid sequences.
The method incorporates a prediction of cleavage sites and a signal peptide/non-signal peptide prediction
based on a combination of several artificial neural networks and hidden Markov models.

Neural networkHMM
Smean scoreSmean predictionSmax scoreSmax predictionD-scoreD predictionHMM prediction
0.082NO0.157NO0.102NON

TargetP

TargetP 1.1 predicts the subcellular location of eukaryotic proteins. The location assignment is based on
the predicted presence of any of the N-terminal presequences: mitochondrial targeting peptide (mTP)
or secretory pathway signal peptide (SP).

Mitochondrion ScoreSecretory ScoreAny other locationLocalizationReliability classPredicted presenquence length
0.7270.0240.35M4119

TMHMM

Prediction of transmembrane helices in proteins

ExpAA=0.00016First60=0.00016PredHel=0
Topology=()

ExpAA: The expected number of amino acids intransmembrane helices.
First60: The expected number of amino acids in transmembrane helices in the first 60 amino acids of the protein.
PredHel: The number of predicted transmembrane helices by N-best.
Topology: The topology predicted by N-best.

Sequence:
>GSTUMT00010022001
MTSTRRQSLFETRHLSRPSLADDIILGPPKMMFASSSTRIPSGKFDHDSH
TTSLASPVIDEDRESSRFERFRMGGGIRLGKEMDKDRPERSSLRGDHTKR
GFGRDDAEGWTSVSRQPRKSFGAEDGDRFRGAGDRGKRENPWDKDRPPKY
DNFGREREHRPRGRRDEASWLLEDRGPGNEDKTGDSKTVHSMEEFQRWKE
RMKASNAPEEKKIKVQAPGELVGQDKFPKEPKGDQQSPKETKDTHFAIEE
PATAPAVVENGVDRFFGLWGAPSSSKSPEQDILGNARPETKAAVKSRFTS
FFAPQEPISPDIPEPPVATKIPTPANESSSEDKEGFQRILKILSGVSLGS
SEPVSVPSPGMSRPPQPSVSPLASMMASHPPPPHSQQTPVTSPPHRDANA
DFLMRLMHQSHQPQQNQAQGPPPPPPLHTSYGGPSQMPPPPPISTSPHNF
QRAKNNPLSPAFDDPAITAFRQRSSQESTSPILPRGPPLIEQHGPPGGWN
HHHQQQQQLSQQQQQPQQPPPQNHRQVAPPPGFPRPQHQQQHHQGPPPPP
PFLGPPPGVNGPPPMPFALGPPPLSMPPNFGPPPPFFGMNGMNGLNSHPP
PPPPPQMNGPPSQGYHIPFHHPHAEPHGPPMGMHGPPFGFPHDGLKHPQH
PHGPGNPRFPGEPLHRQLR

EST contigs matching with the Gene:

ContigBest BlastXE-value
Contig2689 No hit found
SY0AAB37YC14FM1 predicted protein [Ajellomyces capsulatus NAm1]9e-07


Manual Annotation:

Please find below the necessary files for GSTUMT00010022001 annotation :

link to GSTUMT00010022001 Artemis file
link to scaffold_65 sequence file
link to scaffold_65ESTs