Tuber melanosporum DB

You are here: MycorWeb Genome resources Tuber melanosporum DB EST & Gene Sequences Gene card

Gene Name: GSTUMT00006258001
Length(AA) : 1001
Localization : scaffold_25
Number of EST contigs matching the gene: 2
Number of ESTs : 3
  User annotation : No user annotation

Blast Results:

Nr Definition: conserved hypothetical protein
Specie: [Aspergillus terreus NIH2624]
Accession: ref|XP_001213851.1| Score: 1509 E_value: 1e-165
KEGG Definition: DNA-binding proteins Bright/BRCAA1/RBP1 and related proteins containing BRIGHT domain
Accession: aor:AO090003000691 Score: 1459 E_value: 1e-159
KOG Definition: Vesicle coat complex COPII
Classification: [
Kog Number: KOG0307 Score: 181 E_value: 1e-13
PFAM Definition: Atrophin-1, Atrophin-1 family. Atrophin-1 is the protein product of the dentatorubral-pallidoluysian atrophy (DRPLA) gene. DRPLA OMIM:125370 is a progressive neurodegenerative disorder. It is caused by the expansion of a CAG repeat in the DRPLA gene on chromosome 12p. This results in an extended polyglutamine region in atrophin-1, that is thought to confer toxicity to the protein, possibly through altering its interactions with other proteins. The expansion of a CAG repeat is also the underlying defect in six other neurodegenerative disorders, including Huntington's disease. One interaction of expanded polyglutamine repeats that is thought to be pathogenic is that with the short glutamine repeat in the transcriptional coactivator CREB binding protein, CBP. This interaction draws CBP away from its usual nuclear location to the expanded polyglutamine repeat protein aggregates that are characteristic of the polyglutamine neurodegenerative disorders. This interferes with CBP-mediated transcription and causes cytotoxicity..
Pfam Number: pfam03154 Score: 171 E_value: 1e-12
Swissprot: O74365|SOL1_SCHPO
Gene Ontology:
GO:0000790 = Cellular component: nuclear chromatin
GO:0016514 = Cellular component: SWI/SNF complex
GO:0006357 = Biological process: regulation of transcription from RNA polymerase II promoter

SignalP

SignalP 3.0 predicts the presence and location of signal peptide cleavage sites in amino acid sequences.
The method incorporates a prediction of cleavage sites and a signal peptide/non-signal peptide prediction
based on a combination of several artificial neural networks and hidden Markov models.

Neural networkHMM
Smean scoreSmean predictionSmax scoreSmax predictionD-scoreD predictionHMM prediction
0.115NO0.307NO0.083NON

TargetP

TargetP 1.1 predicts the subcellular location of eukaryotic proteins. The location assignment is based on
the predicted presence of any of the N-terminal presequences: mitochondrial targeting peptide (mTP)
or secretory pathway signal peptide (SP).

Mitochondrion ScoreSecretory ScoreAny other locationLocalizationReliability classPredicted presenquence length
0.1620.0830.798_20

TMHMM

Prediction of transmembrane helices in proteins

ExpAA=0.01402First60=0PredHel=0
Topology=()

ExpAA: The expected number of amino acids intransmembrane helices.
First60: The expected number of amino acids in transmembrane helices in the first 60 amino acids of the protein.
PredHel: The number of predicted transmembrane helices by N-best.
Topology: The topology predicted by N-best.

Sequence:
>GSTUMT00006258001
MSVPSAPGWLQDAPAVQNSTFAGPEASSVTAMHSPFDPGAAVITPSTIDP
HQFQQQAHPRAQIQNGNPRTSSPGFQQSAYNVNPVVPLKRPRPREDPITV
SPRQTTANPPTSRSQTPHASGPQLAYQIYQSNAGAAQQRQYTPVGYSSHS
HLQHVGSANASPSPVMQNQTYNQPPPKRVATQSPSPFSPAPPQQPFMPQG
SPPPNQVSRVSTPQNSNMNLAPPPQTPYSQSFTQAFSPPSTAASAPPTPV
PNQQMQNPAQAHMMYQLRTPPHVGPNSMNPGQIPRPQGQQPLQGSGSNPS
QPHSDPNHFMKSLYNFMSKNGTPIQGQSYIGGRAVSLVNLYTNVLKLGGS
QKVTMENKWHAVSMSLGFSPESFPTAADDLVQIFKLNLSAYEQAWLRDRR
DPKKVAIARGQQSGPDMRQQMHPQQQTKPMPNPQQMQHTRPQGMPKQPQT
PVPNNHARSNLHNGFIPQPRQPSRQPPQYSQLPSTPNRISLDSPPQGHIP
PNYPLSAPQIPVKPSVLPDAIPFSQPQNNALKEIPSDFKPKVRGLDTHGG
LNVSVLSQIGNDFMFHKPTTPTFQELGVIDVHALTMMLKCGISAEVRLAL
DTLATVSVESRWSLALESCEDLIETLVDVAESQVEILGEHAEESSEAIAV
PAYEDIVRACKAETVGLIDVARFGSLEHELEKSADKLICITTIFRNLSFF
ESNHKALADPNVVRFLSGVIERLGTSKLLLRTNTNTLDFMKDIIIFLSNL
SHSITLPSEDEALNLLHLILAFAPQPLPAASGRDTLMFTSYSPSIHRYLP
PAVDSLAKLLARDEPNRAFYRTIFLSESSTTPSYDLLTRSFALAISPIPD
NMKVALPQAVEARKPFLEQGMLAAEILAGIAPGPDQGVARSWLSSEDGFA
LSLLRLVCLLSPQVTPMHPRQSGQTGKMPNLDDSQPFARITHRGMGVLQK
LAEKSGDAAGDHSKIPLGVLPKKESLLGALLTVQIDGVIVKKLCTYAGLN
G

EST contigs matching with the Gene:

ContigBest BlastXE-value
Contig4916 hypothetical protein CIMG_03690 [Coccidioides immitis RS]8e-30
SY0AAD57YF06FM1 conserved hypothetical protein [Aspergillus terreus NIH2624]2e-35


Manual Annotation:

Please find below the necessary files for GSTUMT00006258001 annotation :

link to GSTUMT00006258001 Artemis file
link to scaffold_25 sequence file
link to scaffold_25ESTs