Tuber melanosporum DB

You are here: MycorWeb Genome resources Tuber melanosporum DB EST & Gene Sequences Gene card

Gene Name: GSTUMT00003597001
Length(AA) : 441
Localization : scaffold_182
Number of EST contigs matching the gene: 2
Number of ESTs : 41
  User annotation : No user annotation

Blast Results:

Nr Definition: hypothetical protein SNOG_14447
Specie: [Phaeosphaeria nodorum SN15]
Accession: ref|XP_001804633.1| Score: 1092 E_value: 1e-117
KEGG Definition: hypothetical protein; K07300 Ca2+:H+ antiporter
Accession: ani:AN0471.2 Score: 1043 E_value: 1e-111
KOG Definition: Ca2+/H+ antiporter VCX1 and related proteins
Classification: [Inorganic ion transport and metabolism].
Kog Number: KOG1397 Score: 1219 E_value: 1e-134
PFAM Definition: Na_Ca_ex, Sodium/calcium exchanger protein. This is a family of sodium/calcium exchanger integral membrane proteins. This family covers the integral membrane regions of the proteins. Sodium/calcium exchangers regulate intracellular Ca2+ concentrations in many cells; cardiac myocytes, epithelial cells, neurons retinal rod photoreceptors and smooth muscle cells. Ca2+ is moved into or out of the cytosol depending on Na+ concentration. In humans and rats there are 3 isoforms; NCX1 NCX2 and NCX3..
Pfam Number: pfam01699 Score: 293 E_value: 5e-27
Swissprot: Q99385|VCX1_YEAST
Gene Ontology:
GO:0000324 = Cellular component: vacuole
cell cycle-correlated morphology
GO:0015085 = Molecular function: calcium ion transmembrane transporter activity
GO:0006816 = Biological process: calcium ion transport
GO:0006874 = Biological process: cellular calcium ion homeostasis

SignalP

SignalP 3.0 predicts the presence and location of signal peptide cleavage sites in amino acid sequences.
The method incorporates a prediction of cleavage sites and a signal peptide/non-signal peptide prediction
based on a combination of several artificial neural networks and hidden Markov models.

Neural networkHMM
Smean scoreSmean predictionSmax scoreSmax predictionD-scoreD predictionHMM prediction
0.021NO0.075NO0.022NON

TargetP

TargetP 1.1 predicts the subcellular location of eukaryotic proteins. The location assignment is based on
the predicted presence of any of the N-terminal presequences: mitochondrial targeting peptide (mTP)
or secretory pathway signal peptide (SP).

Mitochondrion ScoreSecretory ScoreAny other locationLocalizationReliability classPredicted presenquence length
0.0770.250.89_20

TMHMM

Prediction of transmembrane helices in proteins

ExpAA=230.24First60=0.00789PredHel=10
Topology=(63-85,92-111,121-143,156-175,190-212,225-242,311-333,345-367,372-394,401-420)

ExpAA: The expected number of amino acids intransmembrane helices.
First60: The expected number of amino acids in transmembrane helices in the first 60 amino acids of the protein.
PredHel: The number of predicted transmembrane helices by N-best.
Topology: The topology predicted by N-best.

Sequence:
>GSTUMT00003597001
MPPFGDIEEGRPSRQNSERGPLLDGPNGNGYAAGYGDANGRIAPRSQGPR
RGGFGSHVCSKTKAILLSNYVNTLLIFVPLGIVAGAMKWNSTAIFVMNFL
AIIPLAALLSFATEELALMVGETLGGLLNATFGNAVELIVSIVALTKGEI
RIVQASMLGSILSNILLVLGMCFFFGGLKHKEQNFNTTVASTMSSLMAVA
SSSLIIPAALYSTLAAPKEASFKLILDLSRGTSVVLLILYVLYLVFQLDT
HAYLFDAQHESGESDDDEPQGQQTALLSGVESGVVLVVVTVMVAVCAEYL
VGSIDEIVASSGISKTFIGLILIPIVGNAAEHVTAVVVAMKNKMDLAIGV
AIGSSMQIALLVTPFLVILGWIIGQPMSLYFQTFETVVFFVSVLITNYLI
QDGKSNYLEGAMLLGIYTIIAISFFVYPDSAGSLGGVPGQS

EST contigs matching with the Gene:

ContigBest BlastXE-value
Contig1660 hypothetical protein SNOG_14447 [Phaeosphaeria nodorum SN15]1e-117
SY0AAB56YG22FM1 hypothetical protein SNOG_14447 [Phaeosphaeria nodorum SN15]1e-11


Manual Annotation:

Please find below the necessary files for GSTUMT00003597001 annotation :

link to GSTUMT00003597001 Artemis file
link to scaffold_182 sequence file
link to scaffold_182ESTs