Tuber melanosporum DB

You are here: MycorWeb Genome resources Tuber melanosporum DB EST & Gene Sequences Gene card

Gene Name: GSTUMT00002000001
Length(AA) : 593
Localization : scaffold_333
Number of EST contigs matching the gene: 1
Number of ESTs : 1
  User annotation : No user annotation

Blast Results:

Nr Definition: hypothetical protein NCU05253
Specie: [Neurospora crassa OR74A]
Accession: ref|XP_961790.1| Score: 314 E_value: 1e-26
KEGG Definition: hypothetical protein
Accession: ncr:NCU05253.1 Score: 314 E_value: 5e-27
KOG Definition: Actin regulatory protein (Wiskott-Aldrich syndrome protein)
Classification: [Signal transduction mechanisms
Kog Number: KOG3671 Score: 147 E_value: 5e-10
PFAM Definition: Atrophin-1, Atrophin-1 family. Atrophin-1 is the protein product of the dentatorubral-pallidoluysian atrophy (DRPLA) gene. DRPLA OMIM:125370 is a progressive neurodegenerative disorder. It is caused by the expansion of a CAG repeat in the DRPLA gene on chromosome 12p. This results in an extended polyglutamine region in atrophin-1, that is thought to confer toxicity to the protein, possibly through altering its interactions with other proteins. The expansion of a CAG repeat is also the underlying defect in six other neurodegenerative disorders, including Huntington's disease. One interaction of expanded polyglutamine repeats that is thought to be pathogenic is that with the short glutamine repeat in the transcriptional coactivator CREB binding protein, CBP. This interaction draws CBP away from its usual nuclear location to the expanded polyglutamine repeat protein aggregates that are characteristic of the polyglutamine neurodegenerative disorders. This interferes with CBP-mediated transcription and causes cytotoxicity..
Pfam Number: pfam03154 Score: 143 E_value: 1e-09
Swissprot: No Hit Found

SignalP

SignalP 3.0 predicts the presence and location of signal peptide cleavage sites in amino acid sequences.
The method incorporates a prediction of cleavage sites and a signal peptide/non-signal peptide prediction
based on a combination of several artificial neural networks and hidden Markov models.

Neural networkHMM
Smean scoreSmean predictionSmax scoreSmax predictionD-scoreD predictionHMM prediction
0.216NO0.516NO0.161NON

TargetP

TargetP 1.1 predicts the subcellular location of eukaryotic proteins. The location assignment is based on
the predicted presence of any of the N-terminal presequences: mitochondrial targeting peptide (mTP)
or secretory pathway signal peptide (SP).

Mitochondrion ScoreSecretory ScoreAny other locationLocalizationReliability classPredicted presenquence length
0.4630.0260.549_50

TMHMM

Prediction of transmembrane helices in proteins

ExpAA=0.02017First60=0.00037PredHel=0
Topology=()

ExpAA: The expected number of amino acids intransmembrane helices.
First60: The expected number of amino acids in transmembrane helices in the first 60 amino acids of the protein.
PredHel: The number of predicted transmembrane helices by N-best.
Topology: The topology predicted by N-best.

Sequence:
>GSTUMT00002000001
MGIRRLYTNLRPYARPTILEPCTEIFIDGPGLAHHVYEKCYSAAPPARPE
DVNPWDSAIPYSVLQDSVIKYLHMLESCGAKMIYFDGALPASKRPTRILR
LEESLRKLCTFHASYSHTLPRCSDRSAREWGGVEIFSERAVYTPQSPVLS
TPPFLVTATLAALSRGGYAERTSTVPGEAEGYAARDAIAAGGVVLTSDSD
LLLFRGEGENDWGVVMFKDLEVNFSTGQISTVLFRPEDIAKGFDLELAFL
GYLSLCNPQATFSTLREWCARITERSRSGAIVEEMGRKWREFAAGYALPE
GDGGRLIDPRIGELLSLRGAVGRKREMFLPTLWEDTSRASAWDVGREVRA
LAYSLLFPGEMSLEEISRRGHGISGAPVEIILGRKAKQKLHEIILESQEE
DWQARYVLQQIVKTFAARNKPPPHASILESSASLVSACAHTSTDATNRQT
AGVRWTWERIQTFAMLQAAWYSLLLLSEVLKETTSSKSGLGVLPVEVLDG
RRFVDARPGAGAKSLVKEILAAYFGEEEEEEEEEEEDMKARRRMSKEENL
EGVWKEASGKARRKRRWPAEEVAEVKVKVAATKGGNMFAALVE

EST contigs matching with the Gene:

ContigBest BlastXE-value
SY0AAB14YP22FM1 hypothetical protein NCU05253 [Neurospora crassa OR74A]3e-07


Manual Annotation:

Please find below the necessary files for GSTUMT00002000001 annotation :

link to GSTUMT00002000001 Artemis file
link to scaffold_333 sequence file
link to scaffold_333ESTs