Posts Tagged ‘symbiont’

Basidiomycete Genome Meeting

December 19th, 2010

FomitopsisIgor Grigoriev, David Hibbett and I are organizing a one-day workshop on March 21, 2011 at the JGI in Walnut Creek to cover comparative genome analysis of basidiomycetes. This jamboree will take place between the Fungal Genetics Conference in Asilomar (Mar 15-20) and the JGI User Meeting (Mar 22-24). The workshop will  include sessions on wood decayers, symbionts and soil fungal metagenomics.

The various sessions will include an update on current genome sequencing projects running at the JGI, including the Wood Decayers and Mycorrhizal Symbionts projects, and cross-all-basidio comparisons.

People willing to make a contribution can contact Igor, David or myself.

Photo: Early stages of Fomitopsis fruiting body development. Spruce forest near Pierre Percée in the Vosges range. © F Martin

One Bacterial Cell, One Complete Genome

May 1st, 2010

90% of microbial bugs are eluding current culturing attempts. Sequencing of single cells is a novel culture-independent approach, which enables access to the genetic material of an individual cell of unculturable bacteria. In PLoS One this week, Jan-Fang Cheng’s and Nancy Moran’s groups at JGI and the University of Arizona report the completed sequence of Candidatus Sulcia meulleri, obtained from an uncultured single cell. The Bacteroidetes Sulcia is one of two obligate bacterial symbionts inhabiting sharpshooters. A single Sulcia cell was sampled from the host bacteriome using an inverted microscope (Zeiss) and a micromanipulator, its genome amplified via multiple displacement amplification and sequenced using a combination of Sanger sequence and pyrosequencing, generating a total of 57 Mb of sequence. This approach can now be used to generate complete reference genomes urgently needed for metagenomic of bacterial communities.