The tree-killing fungus Grosmannia clavigera (= Ophiostoma clavigerum) carried by the mountain pine beetle (MPB), Dendroctonus ponderosae, is devastating pine forests in British Columbia (Canada). This sap-stain ascomycetous fungus grows rapidly in the host tree phloem and through the sapwood where it produces melanin that discolours the wood, and blocks the host tree’s water transport system. More than 15 million hectares of pine forests are currently affected by MPB. The pathogenic fungus recently crossed the Rocky Mountains into Alberta, raising the concern that pine forests across Canada may become affected. Bark beetles, beetle-associated tree-infecting fungi, and pine trees are three of the major interacting biological components of this epidemic. Genomics offers new approaches to delineate some of these complex biological interactions (see the TRIA project web site for details). Scott DiGuistini and his collaborators have combined combined conventional, 40-kb fosmid paired-end Sanger reads from an ABI 3730xl sequencer, single-end 454 reads from Roche GS20 and GS FLX sequencers, and paired-end reads from an Illumina Genome Analyzer sequencer for generating the ~32.5 Mb draft genome sequence of G. clavigera. They developed a hybrid approach that uses the Forge and Velvet assemblers for generating the de novo draft genome sequences. This G. clavigera genome sequencing, together with the sequencing of the methylotrophic yeast Pichia pastoris, demonstrate that the new sequencing technologies can efficiently be used for generating genome draft for eukaryotic fungal genomes.