Tuber melanosporum DB

You are here: MycorWeb Genome resources Tuber melanosporum DB EST & Gene Sequences Gene card

Gene Name: GSTUMT00009579001
Length(AA) : 1200
Localization : scaffold_59
Number of EST contigs matching the gene: 2
Number of ESTs : 6
                         
  User annotation : No user annotation

Blast Results:

Nr Definition: hypothetical protein HCAG_04084
Specie: [Ajellomyces capsulatus NAm1]
Accession: ref|XP_001540244.1| Score: 1452 E_value: 1e-158
KEGG Definition: SET domain protein [EC:2.1.1.43]; K05302 histone-lysine N-methyltransferase
Accession: afm:AFUA_6G06335 Score: 1446 E_value: 1e-158
KOG Definition: Histone H3 (Lys4) methyltransferase complex, subunit SET1 and related methyltransferases
Classification: [Chromatin structure and dynamics, Transcription].
Kog Number: KOG1080 Score: 674 E_value: 9e-71
PFAM Definition: SET, SET domain. SET domains are protein lysine methyltransferase enzymes. SET domains appear to be protein-protein interaction domains. It has been demonstrated that SET domains mediate interactions with a family of proteins that display similarity with dual-specificity phosphatases (dsPTPases). A subset of SET domains have been called PR domains. These domains are divergent in sequence from other SET domains, but also appear to mediate protein-protein interaction. The SET domain consists of two regions known as SET-N and SET-C. SET-C forms an unusual and conserved knot-like structure of probably functional importance. Additionally to SET-N and SET-C, an insert region (SET-I) and flanking regions of high structural variability form part of the overall structure..
Pfam Number: pfam00856 Score: 371 E_value: 1e-35
Swissprot: Q4WNH8|SET1_ASPFU
Gene Ontology:
GO:0018024 = Molecular function: histone-lysine N-methyltransferase activity

SignalP

SignalP 3.0 predicts the presence and location of signal peptide cleavage sites in amino acid sequences.
The method incorporates a prediction of cleavage sites and a signal peptide/non-signal peptide prediction
based on a combination of several artificial neural networks and hidden Markov models.

Neural networkHMM
Smean scoreSmean predictionSmax scoreSmax predictionD-scoreD predictionHMM prediction
0.092NO0.315NO0.110NON

TargetP

TargetP 1.1 predicts the subcellular location of eukaryotic proteins. The location assignment is based on
the predicted presence of any of the N-terminal presequences: mitochondrial targeting peptide (mTP)
or secretory pathway signal peptide (SP).

Mitochondrion ScoreSecretory ScoreAny other locationLocalizationReliability classPredicted presenquence length
0.1280.0970.779_20

TMHMM

Prediction of transmembrane helices in proteins

ExpAA=0.0038First60=0.00006PredHel=0
Topology=()

ExpAA: The expected number of amino acids intransmembrane helices.
First60: The expected number of amino acids in transmembrane helices in the first 60 amino acids of the protein.
PredHel: The number of predicted transmembrane helices by N-best.
Topology: The topology predicted by N-best.

Sequence:
>GSTUMT00009579001
MSRAGYADFFPAAPSVLAEKEKKRAQAERQDRRARNVPDDYDRESNAISV
SDPGHIHSSSCAPISVGVSLPSGHLVTTSTLTPTTTSSSPPPPLLSPSAG
TTAKGDYPPNKLAAQSTSAREPYPLPPPPIPPPLAPLTPKGTPPAIKSDP
SRRNWKVKYDPFLEKKSTTRKSREPILRWDGEGITTPVTDPRLEVGRYTD
GPKTGKKRLRSQGLYRTRWKWDENYIGPGPPSQILITGLSPLTTETEISM
HFRPHGDIQTLEIRIDPATGGSLGICSIIYRDGPRSRSPAHEAAKRAAQE
VNGSRVGIQRVRVELDRDGLKCKKLTGRILEEKRRKEAEAEKNRLKQPLN
ISYGELGSRPRSPSARGSSPSRRDSRIDGRSDDRSRDQRPTPAPRPDNYR
ALDHVGRLPYIFITSRCIPGDERYVRHLNGRLKNFGLKDILWDRTGFYVV
FWERRDMERCFKVCDGDRLFSYTMIMEKHPGSNRSPSPPPVARPELRKMG
PNELVVEATNALMKDMKLVLMKDLRKRLAAPTIYDSLEVGRFNKRRKVEE
EEVQGITAGSHQVLAVSLEASIRSPTTLLPTLALKSTEKLTSAVAKSALQ
KPTALPRFKKRILTKAEDSSTPEASPLLHGSRTRLSKADARPLAHRLNQY
NSDAGSDDESTITENRPVSRGSRALSLDTGDDDSVSVALSANEGYRKRKR
SVGPTSKLRDTAVSTDDEDHSLEKDDGSQGLHDLKKPRIDHPRPYEDGEV
FMGDEVDEEPAVATPHRTSTTTPQLKPITKNKSRTIESDSEYYEDEMVFT
KADPKDESELVNVLDDEDDRGPEIEIPNRTTLSREPRKRIKTTESRTNRD
LLAVPSFDADEEKHDSEMAGMDIEVSWGVSTTEGPMVTVQDDPDAILDID
SLQNLVKDAEDFKFLAQALAAVEPADLGNVQAWAYKEKQLKVAQKQSIAR
SQEKIEGFYRPNPTGCARTEGYRRVPEAEKSMYLPHRLQLAAKVKSAANK
IPEVTAAAVTNPTKNPSSTSRMNRVNNRRLVVGLNNQKQMLSSDADVMRF
NQLKKRKKPVKFARSAIHNWGLYAMENISAGDMIIEYVGEIIRQQVADMR
EKKYLKSGIGSSYLFRIDDTTVIDATKAGGIARFINHSCTPNCTAKIIKV
EGSKRIVIYALRDIRENEELTYDYKFERELESEERIPCLCGSSGCKGFLN

EST contigs matching with the Gene:

ContigBest BlastXE-value
Contig7441 hypothetical protein AN5795.2 [Aspergillus nidulans FGSC A4]4e-20
Contig7586 hypothetical protein HCAG_04084 [Ajellomyces capsulatus NAm1]6e-39


Manual Annotation:

Please find below the necessary files for GSTUMT00009579001 annotation :

link to GSTUMT00009579001 Artemis file
link to scaffold_59 sequence file
link to scaffold_59ESTs