Jean-Michel Girardet

PAST AND CURRENT FUNCTIONS:

– 2015: Currently assigned to UMR 1136 INRA/University of Lorraine IAM as Research Engineer.

– 1993-2014: Engineer in Food Biochemistry assigned to UR AFPA, USC INRA 340, University of Lorraine.

QUALIFICATIONS:

– 2012: H.D.R. (Qualified Research Supervisor) from the University of Lorraine, France: ‘Technofunctional and Biofunctional Properties of Milk Proteins and Peptides’.

– 1992: Ph.D. in Applied Biochemistry from the University of Nancy 1, France : ‘Component PP3 of Milk: Preparation, Origin, Study of its Glycan Moiety, Role in the Inhibition of Lipolysis’. (INRA-Region of Lorraine scholarship)

Ph.D. SUPERVISOR COMMITTEE:

-AHMAD Nazir: ‘Postprandial lipemia and lactoferrin: The Lipolysis Stimulated Lipoprotein Receptor as potential target’ December 2012.

-MATÉOS Aurélie: ‘Proteomic study of equine alphas1– and beta-caseins microheterogeneity: Identification of posttranscriptional variants and phosphorylation variants; identification of the phosphorylation sites of the equine beta-casein’ November 2008.

-N’NEGUE Marie-Andrée: ‘Susceptibility of alpha-lactalbumin towards proteolysis in relationship to the conformational changes induced by thermal treatments. Identification of alpha-lactalbumin peptides possessing antioxidant activity’ June 2006.

-DO EGITO Antonio Silvio: ‘Equine milk proteins: Physico-chemical properties and highlighting of Angiotensin I Converting Enzyme (ACE) inhibitory peptides’ April 2002.

 CURRENT ADMINISTRATIVE DUTIES:

– Elected member of staff (CPE) at the University of Lorraine (ca. 3000 technicians and engineers concerned).

– Appointed as expert in juries in national competitions for the recruitment of staff.

– Elected member at the scientifc council of UMR IAM.

 PEER-REVIEWED PUBLICATIONS:

Mati A., Senoussi-Ghezali C., Si Ahmed Zennia S., Almi-Sebbane D., El-Hatmi H. & Girardet J.M. (2017). Dromedary camel milk proteins, a source of peptides having biological activities – A review. International Dairy Journal, 73, 25-37.

Schwartz M., Didierjean C., Hecker A., Girardet J.M., Morel-Rouhier M., Gelhaye E. & Favier F. (2016). Crystal structure of Saccharomyces cerevisiae ECM4, a Xi-class glutathione transferase that reacts with glutathionyl-(hydro)quinones. PLOS One, 11, e0164678.

Lallement P.A., Roret T., Tsan P., Gualberto J.M., Girardet J.M., Didierjean C., Rouhier N. & Hecker A. (2016). Insights into ascorbate regeneration in plants: Investigating the redox and structural properties of dehydroascorbate reductases from Populus trichocarpaBiochemical Journal, 473, 717-731.

Guerin J., Kriznik A., Ramalanjaona N., Le Roux Y. & Girardet J.M. (2016). Interaction between dietary bioactive peptides of short length and bile salts in submicellar or micellar state. Food Chemistry, 209, 114-122.

El Hatmi H., Jard Z., Khorchani T., Jardin J., Poirson C., Perrin C., Cakir-Kiefer C. & Girardet J.M. (2016). Identification of bioactive peptides derived from caseins, Glycosylation-Dependent Cell Adhesion Molecule-1 (GlyCAM-1), and Peptidoglycan Recognition Protein-1 in fermented camel milk. International Dairy Journal56, 159-168.

Hafeez Z., Cakir-Kiefer C., Girardet J.M., Lecomte X., Paris C., Galia W., Dary A. & Miclo L. (2015). New Insights into the proteolytic system of Streptococcus thermophilus: Use of isracidin to characterize cell-associated extracellular peptidase activities. Journal of Agricultural and Food Chemistry, 63, 7522-7531.

Si Ahmed Zennia S., Mati A., Saulnier F., Verdier Y., Chiappetta G., Mulliert G., Miclo L., Vinh J. & Girardet J.M. (2015). Identification by FT-ICR-MS of Camelus dromedarius alpha-lactalbumin variants as the result of nonenzymatic deamidation of Asn-16 and Asn-45. Food Chemistry, 187, 305-313.

Canabady-Rochelle L.L.S., Harscoat-Schiavo C., Kessler V., Fournier F., Aymes A. & Girardet J.M. (2015). Determination of reducing power and metal chelating ability of antioxidant peptides: Revisited Methods. Food Chemistry, 183, 129-135.

Jrad Z., Girardet J.M., Adt I., Oulahal N., Degraeve P., Khorchani T. & El Hatmi H. (2014). Antioxidant activity of camel milk casein before and after in vitro simulated enzymatic digestion. Mljekarstvo, 64, 287-294.

Jrad Z., El Hatmi H., Adt I., Girardet J.M., Cakir-Kiefer C., Jardin J., Degraeve P., Khorchani T. & Oulahal N. (2014). Effect of digestive enzymes on antimicrobial, radical scavenging and angiotensin I-converting enzyme inhibitory activities of camel colostrum and milk proteins. Dairy Science and Technology, 94, 205-224.

Bunet R., Riclea R., Laureti L., Hôtel L., Paris C., Girardet J.M., Spiteller D., Dickschat J.S., Leblond P. & Aigle B. (2014). A single Sfp-type phosphopantetheinyl transferase plays a major role in the biosynthesis of PKS and NRPS derived metabolites in Streptomyces ambofaciens ATCC23877. PLOS One, 9, e87607.

El Hatmi H., Z. Jrad Z., Khorchani T., Dary A. & Girardet J.M. (2014). Fast protein liquid chromatography of camel alpha-lactalbumin fraction with radical scavenging activity. Emirates Journal of Food and Agriculture, 26, 309-316.

Zidane F., Zeder-Lutz G., Altschuh D., Girardet J.M., Miclo L., Corbier C. & Cakir-Kiefer C. (2013). Surface plasmon resonance analysis of the binding mechanism of pharmacological and peptidic inhibitors to human somatic angiotensin I-converting enzyme. Biochemistry, 52, 8722-8731.

Hafeez, Z., Cakir-Kiefer, C., Girardet, J.M., Jardin, J., Perrin, C., Dary, A., & Miclo, L. (2013). Hydrolysis of milk-derived bioactive peptides by cell-associated extracellular peptidases of Streptococcus thermophilus. Applied Microbiology and Biotechnology, 97, 9787-9799.

Ahmad N., Girardet J.M., Akbar S., Lanhers M.-C., Paris C., Yen F.T. & Corbier C. (2012). Lactoferrin and its hydrolysate bind directly to the oleate-activated form of the lipolysis stimulated lipoprotein receptor. FEBS Journal, 279, 4361-4373.

Zidane F., Matéos A., Cakir-Kiefer C., Miclo L., Rahuel-Clermont S., Girardet J.M. & Corbier C. (2012). Binding of divalent metal ions to 1-25 beta-caseinophosphopeptide: an isothermal titration calorimetry study. Food Chemistry, 132, 391-398.

Sadat L., Cakir-Kiefer C., N’Negue M.A., Gaillard J.L., Girardet J.M. & Miclo, L. (2011). Isolation and identification of antioxidative peptides from bovine alpha-lactalbumin. International Dairy Journal, 21, 214-221.

Matéos A., Girardet J.M., Mollé D., Corbier C., Gaillard J.L. & Miclo L. (2010). Identification of phosphorylation sites of equine beta-casein isoforms. Rapid Communications in Mass Spectrometry, 24, 1533-1542.

Matéos A., Miclo L., Mollé D., Dary A., Girardet J.M. & Gaillard J.L. (2009). Equine alphas1-casein : characterization of alternative splicing isoforms and determination of phosphorylation levels. Journal of Dairy Science, 92, 3604-3615.

Matéos A., Girardet J.M., Mollé D., Dary A., Miclo L. & Gaillard J.L. (2009). Two-dimensional cartography of equine beta-casein variants achieved by isolation of phosphorylation isoforms and control of the deamidation phenomenon. Journal of Dairy Science, 92, 2389-2399.

Miclo L., Girardet J.M., Egito A.S., Mollé D., Martin P. & Gaillard J.L. (2007). The primary structure of a low-Mr multi-phosphorylated variant of beta-casein in equine milk. Proteomics 7, 1327-1335.

Egito A.S., Girardet J.M., Laguna L.E., Poirson C., Mollé D., Miclo L., Humbert G. & Gaillard J.L. (2007). Milk-clotting activity of enzyme extracts from sunflower and albizia seeds and specific hydrolysis of kappa‑casein. International Dairy Journal, 17, 816-825.

El-Hatmi H., Girardet J.M., Gaillard J.L., Yahyaoui M.H. & Attia H. (2007). Characterization of whey proteins of camel (Camelus dromedarius) milk and colostrum. Small Ruminant Research , 70, 267-271.

Egito A.S., Rosinha G.M.S., Laguna L.E., Miclo L., Girardet J.M. & Gaillard J.L. (2006). [Fast electrophoretic detection method of adulteration of caprine milk by bovine milk]. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, 58, 932-939.

Girardet J.M., Miclo L., Florent S., Mollé D. & Gaillard J.L. (2006). Determination of the phosphorylation level and deamidation susceptibility of equine beta-casein. Proteomics, 6, 3707-3717.

N’Negue M.A., Miclo L., Girardet J.M., Campagna S., Mollé D. & Gaillard J.L. (2006). Proteolysis of bovine alpha-lactalbumin by thermolysin during thermal denaturation. International Dairy Journal, 16, 1157-1167.

Pang X., Aigle B., Girardet J.M., Mangenot S., Pernodet J.L., Decaris B. & Leblond P. (2004). Functional angucycline-like antibiotic gene cluster in the terminal inverted repeats of the Streptomyces ambofaciens linear chromosome. Antimicrobial Agents and Chemotherapy, 48, 575-588.

Nabhan M.A., Girardet J.M., Campagna S., Gaillard J.L. & Le Roux Y. (2004). Isolation and characterization of co-polymers of beta-lactoglobulin, alpha-lactalbumin, kappa-casein, and alphas1-casein generated by pressurization and thermal treatment of raw milk. Journal of Dairy Science, 87, 3614-3622.

Campagna S., Mathot A.G., Fleury Y., Girardet J.M. & Gaillard J.L. (2004). Antibacterial activity of lactophoricin, a synthetic 23-residues peptide derived from the sequence of bovine milk component-3 of proteose peptone. Journal of Dairy Science, 87, 1621-1626.

Girardet J.M., N’Negue M.A., Egito A.S., Campagna S., Lagrange A. & Gaillard J.L. (2004). Multiple forms for equine alpha‑lactalbumin: evidence of N-glycosylated and deamidated forms. International Dairy Journal, 14, 207-217.

Egito A.S., Girardet J.M., Poirson C., Mollé D., Humbert G., Miclo L. & Gaillard J.L. (2003). Action of plasmin on equine beta‑casein. International Dairy Journal, 13, 813-820.

Moussaoui F., Laurent F., Girardet J.M., Humbert G., Gaillard J.L. & Le Roux Y. (2003). Characterization and proteolytic origins of specific peptides appearing during lipopolysaccharide experimental mastitis. Journal of Dairy Science, 86, 1163-1170.

Gelhaye E., Rouhier N., Vlamis-Gardikas A., Girardet J.M., Sautière P.E., Sauzet M., Martin F. & Jacquot J.P. (2003). Identification and characterization of a third thioredoxin h in poplar. Plant Physiology and Biochemistry, 41, 629-635.

Egito A.S., Miclo L., Lopez C., Adam A., Girardet J.M. & Gaillard J.L. (2002). Separation and characterization of mares’ milk alphas1-, beta-, kappa-caseins, gamma-casein-like, and proteose peptone component 5-like peptides. Journal of Dairy Science, 85, 697-706.

Egito A.S., Haussard M., Poirson C., Girardet J.M., Miclo L. & Gaillard J.L. (2002) Angiotensin I-converting enzyme inhibitory properties of an equine tryptic digest and characterisation of some active peptides. In Peptides 2002 pp. 92-93 (Ettore Benedetti and Carlo Pedone, Eds.), Edizioni Ziino, Napoli, Italia.

Egito A.S., Girardet J.M., Miclo L., Mollé D., Humbert G. & Gaillard J.L. (2001). Susceptibility of equine kappa- and beta-caseins to hydrolysis by chymosin. International Dairy Journal, 11, 885-893.

Egito A.S., Girardet J.M., Miclo L. & Gaillard J.L. (2001). Highly sensitive periodic acid/Schiff detection of bovine milk glycoproteins electrotransferred after nondenaturing electrophoresis, urea electrophoresis, and isoelectric focusing. Le Lait, 81, 775-785.

Girardet J.M., Humbert G., Creusot N., Chardot V., Campagna S., Courthaudon J.L. & Gaillard J.L. (2001). Dilational rheology of mixed beta-casein/Tween 20 and beta-casein (f114-169)/Tween 20 films at oil-water interface. Journal of Colloid and Interface Science, 243, 515-522.

Girardet J.M., Debomy L., Courthaudon J.L., Miclo L., Humbert G. & Gaillard J.L. (2000). Viscoelastic properties of oil-water interfaces covered by bovine beta-casein tryptic peptides. Journal of Dairy Science, 83, 2410-2421.

Girardet J.M., Saulnier F., Gaillard J.L., Ramet J.P. & Humbert G. (2000). Camel (Camelus dromedarius) milk PP3: evidence for an insertion in the amino-terminal sequence of the camel milk whey protein. Biochemistry and Cell Biology, 78, 19-26.

Courthaudon J.L., Girardet J.M., Campagne S., Rouhier L.M., Campagna S., Lorient D. & Linden G. (1999). Surface active and emulsifying properties of casein micelles compared to those of sodium caseinate. International Dairy Journal, 9, 411-412.

Girardet J.M., Courthaudon J.L., Campagna S., Puyjalon V., Lorient D. & Linden G. (1999). Proteolysis of milk casein micelle and sodium caseinate in oil-in-water emulsion. International Dairy Journal, 9, 409-410.

Montagne L., Girardet J.M. & Humbert G. (1999). Amino-terminal sequencing of the ovine milk fat globule membrane protein butyrophilin. Le Lait, 79, 275-279.

Campagna S., Vitoux B., Humbert G., Girardet J.M., Linden G., Haertle T. & Gaillard J.L. (1998). Conformational studies of a synthetic peptide from the putative lipid-binding domain of bovine milk component PP3. Journal of Dairy Science, 81, 3139-3148.

Girardet J.M., Saulnier F., Linden G. & Humbert G. (1998). Rapid separation of bovine whey proteins by membrane convective liquid chromatography, perfusion chromatography, continuous bed chromatography, and capillary electrophoresis. Le Lait, 78, 391-400.

Coddeville B., Girardet J.M., Plancke Y., Campagna S., Linden G. & Spik G. (1998), Structures of the O‑glycopeptides isolated from bovine milk component PP3. Glycoconjugate Journal, 15, 371-378.

Guimont C., Marchal E., Girardet J.M. & Linden G. (1997). Biological active factors in bovine milk and dairy by-products: influence on cell culture. Critical Reviews in Food Science and Nutrition, 37, 393-410.

Rudolf E., Girardet J.M., Bautz A.M. & Dournon C. (1997). Purification and partial characterization of peptidase-1, a sex-linked enzyme in Pleurodeles waltl (urodele amphibian). Biochemistry and Cell Biology, 75, 803-806.

Bernos E., Girardet J.M., Humbert G. & Linden G. (1997). Role of the O‑phosphoserine clusters in the interaction of the bovine milk alphas1‑, beta‑, kappa‑caseins and the PP3 component with immobilized iron(III) ions. Biochimica et Biophysica Acta (Protein Structure and Molecular Enzymology), 1337, 149‑159.

Girardet J.M. & Linden G. (1996). PP3 component of bovine milk: a phosphorylated whey glycoprotein. Journal of Dairy Research, 63, 333-350.

Girardet J.M., Coddeville B., Plancke Y., Strecker G., Campagna S. & Linden G. (1995). Structure of glycopeptides isolated from bovine milk component PP3. European Journal of Biochemistry (devenu FEBS Journal), 234, 939-946.

Le Roux Y., Girardet J.M., Humbert G., Laurent F. & Linden G. (1995). Proteolysis in samples of quarter milk with varying somatic cell counts. 2. component PP3 and beta-CN‑1P(f29‑105 and f29‑107) of the proteose-peptone fraction. Journal of Dairy Science, 78, 1298-1305.

Courthaudon J.L., Girardet J.M., Chapal C., Lorient D. & Linden G. (1995) Surface activity and competitive adsorption of milk component-3 and porcine pancreatic lipase at the dodecane-water interface. In Food Macromolecules and Colloids, pp. 58‑70 (Ed. E. Dickinson) London: Royal Society of Chemistry.

Etienne L., Girardet J.M. & Linden G. (1994). Growth promotion of Bifidobacterium animalis by bovine milk proteose-peptone. Le Lait, 74, 313‑323.

Girardet J.M., Saulnier F., Driou A., Linden G., Coddeville B. & Spik G. (1994). High performance electrophoresis chromatography of bovine milk component‑3 glycoproteins. Journal of Dairy Science, 77, 1205‑1216.

Mati A., Moulti-Mati F., Girardet J.M., Fokou E., Belleville-Nabet F., Nabet P. & Linden G. (1993). Mitogenic activity of hydrophobic fractions of proteose-peptone from cows’, ewes’ and goats’ milk measured with MARK 3 hybridoma culture. Journal of Dairy Research, 60, 443‑448.

Girardet J.M., Linden G., Loye S., Courthaudon J.L. & Lorient D. (1993). Study of mechanism of lipolysis inhibition by bovine milk proteose-peptone component‑3. Journal of Dairy Science, 76, 2156‑2163.

Mati A., Girardet J.M., Xenakis D. & Linden G. (1991). Isolement et caractérisation de la fraction hydrophobe des protéose-peptones des laits bovin, ovin et caprin. Le Lait, 71, 259‑273.

Saulnier F., Ferrero F., Choukri A., Girardet J.M. & Linden G. (1991). Estimation de la dénaturation des protéines par spectrofluorimétrie d’absorption moléculaire : applications aux lactosérums industriels. Le Lait, 71, 511‑518.

Girardet J.M., Mati A., Sanogo T., Etienne L. & Linden G. (1991). Fast protein liquid chromatography purification of hydrophobic fraction of bovine milk proteose-peptone and characterization by bidimensional electrophoresis. Journal of Dairy Research, 58, 85‑98.

Girardet J.M., Pâquet D. & Linden G. (1989). Effects of chromatographic parameters on the fractionation of whey proteins by anion exchange FPLC. Milchwissenschaft, 44, 692‑696.

INVITED LECTURES:

Girardet J.M. (2014). Milk proteins as source of biofunctional peptides [Les protéines laitières sources de peptides biofonctionnels]. 1er Congrès International de Technologies Alimentaires et Contrôle Qualité des Aliments “AGROINNOV : Technologies Innovantes pour la Production Alimentaire”, Djerba, Tunisia, April, 25 – 27, 2014.

Girardet J.M. (2011). Antioxidant peptides from milk, unveiling and production by lactic acid bateria [Les peptides antioxydants du lait, leur mise en évidence et leur production par une bactérie lactique]. Antioxydants 2011 : Biodisponibilité, Métabolomique & Innovations Stratégiques. Société Française des Antioxydants (SFA), Paris, France, June, 09, 2011.