Couturier Jérémy

Career

I received my PhD in Forest Biology from University of Lorraine in Nancy in 2007. My research concerned the identification of molecular mechanisms involved in the absorption and transport of different nitrogen sources, ammonium and amino acids, in poplar. Then, I did a post-doctoral research on the elucidation of the functions of glutaredoxins (GRXs) in plants and more particularly on their roles in Fe-S cluster maturation. During this period, I improved my technical skills for the biochemical and structural characterization of proteins involved in the redox regulation.

I have been recruited as an assistant professor in the research unit in September 2013. I am interested by all redox modifications of protein cysteine residues. Besides my involvement in two projects focused on Fe-S cluster maturation and the identification of new metal or redox regulated functions in plants, I am the principal investigator of a project focused on the molecular mechanisms involved in the sulfur trafficking in plants. As a major sulfur donor molecule, cysteine is a key metabolite involved in the biosynthesis of many sulfur-containing cofactors or biomolecules (Fe-S cluster, biotin, thiamine, lipoic acid, molybdopterin and sulfur-containing bases in tRNA). The first hallmark step of sulfur mobilization is the formation of persulfides on a reactive catalytic cysteine of cysteine desulfurases (CDs). Considering the diversity of acceptor molecules, the trafficking of sulfur also implies the existence of carrier proteins, notably of the sulfurtransferase (STR) family. Most STRs catalyze the transfer of a sulfur atom from sulfur donors to nucleophilic sulfur acceptors by forming themselves persulfides. In this context, the intervention of the thioredoxin (TRX) and glutathione/glutaredoxin (GRX) reducing systems in the sulfur signaling/trafficking pathways may be important by modulating this process but it could also provide the required specificity to disseminate the signal from a single persulfide-generating enzyme to specific target proteins acting in this case as secondary carriers.

 

Publications

2018

P37. Gao H, Azam T, Randeniya S, Couturier J, Rouhier N, Johnson MK (2018) Function and maturation of the Fe-S center in dihydroxyacid dehydratase from Arabidopsis. The Journal of Biological Chemistry 293:4422-4433. doi: 10.1074/jbc.RA117.001592.

P36. Przybyla-Toscano J, Roland M, Gaymard F, Couturier J, Rouhier N (2018) Roles and maturation of iron-sulfur proteins in plastids. Journal of Biological Inorganic Chemistry. doi: 10.1007/s00775-018-1532-1

2017

P35. Zannini F, Couturier J, Keech O, Rouhier N (2017) In Vitro Alkylation Methods for Assessing the Protein Redox State. Methods in Molecular Biology 1653:51-64. doi: 10.1007/978-1-4939-7225-8_4.

P34. Przybyla-Toscano J, Roret T, Couturier J, Rouhier N (2017) FeS Cluster Assembly: Role of Monothiol Grxs and Nfu Proteins. Encyclopedia of Inorganic and Bioinorganic Chemistry, Metalloprotein Active Site Assembly. doi: 10.1002/9781119951438.eibc2470.

P33. Selles B, Zannini F, Couturier J, Jacquot JP, Rouhier N (2017) Atypical protein disulfide isomerases (PDI): Comparison of the molecular and catalytic properties of poplar PDI-A and PDI-M with PDI-L1A. PLoS One 12:e0174753. doi: 10.1371/journal.pone.0174753.

P32. Ribeiro CW, Baldacci-Cresp F, Pierre O, Larousse M, Benyamina S, Lambert A, Hopkins J, Castella C, Cazareth J, Alloing G, Boncompagni E, Couturier J, Mergaert P, Gamas P, Rouhier N, Montrichard F, Frendo P (2017) Regulation of Differentiation of Nitrogen-Fixing Bacteria by Microsymbiont Targeting of Plant Thioredoxin s1. Current Biology 27:250-256. doi: 10.1016/j.cub.2016.11.013.

2016

P31. Gütle DD, Roret T, Müller SJ, Couturier J, Lemaire SD, Hecker A, Dhalleine T, Buchanan BB, Reski R, Einsle O, Jacquot JP (2016) Chloroplast FBPase and SBPase are thioredoxin-linked enzymes with similar architecture but different evolutionary histories. Proceedings of the National Academy of Sciences USA 113: 6779-6784. doi: 10.1073/pnas.1606241113.

P30. Jacquot JP, Couturier J, Didierjean C, Gelhaye E, Morel-Rouhier M, Hecker A, Plomion C, Gütle DD, Rouhier N (2016) Structural and functional characterization of tree proteins involved in redox regulation: a new frontier in forest science. Annals of Forest Science 73:119-134. doi: 10.1007/s13595-014-0442-9

 

2015

P29. Rouhier N, Cerveau D, Couturier J, Reichheld JP, Rey P (2015) Involvement of thiol-based mechanisms in plant development. Biochimica and Biophysica Acta 1850:1479-1496. doi: 10.1016/j.bbagen.2015.01.023.

P28. Couturier J, Przybyla-Toscano J, Roret T, Didierjean C, Rouhier N (2015) The roles of glutaredoxins ligating Fe-S clusters: Sensing, transfer or repair functions? Biochimica and Biophysica Acta 1853:1513-1527. doi: 10.1016/j.bbamcr.2014.09.018.

 

2014

P27. Roret T, Pégeot H, Couturier J, Mulliert G, Rouhier N, Didierjean C (2014) X-ray structures of Nfs2, the plastidial cysteine desulfurase from Arabidopsis thaliana. Acta Crystallographica section F Structural Biology Communications 70:1180-1185. doi: 10.1107/S2053230X14017026.

P26. Roret T, Tsan P, Couturier J, Zhang B, Johnson MK, Rouhier N, Didierjean C (2014) Structural and Spectroscopic Insights into BolA-Glutaredoxin Complexes. The Journal of Biological Chemistry 289:24588-24598. doi: 10.1074/jbc.M114.572701.

P25. Dhalleine T, Rouhier N, Couturier J (2014) Putative roles of glutaredoxin-BolA holo-heterodimers in plants. Plant Signaling and Behaviour 9:e28564.

P24. Couturier J, Wu HC, Dhalleine T, Pégeot H, Sudre D, Gualberto JM, Jacquot JP, Gaymard F, Vignols F, Rouhier N (2014) Monothiol Glutaredoxin-BolA Interactions: Redox Control of Arabidopsis thaliana BolA2 and SufE1. Molecular Plant 7:187-205. doi: 10.1093/mp/sst156.

 

2013

P23. Couturier J, Jacquot JP, Rouhier N (2013) Toward a refined classification of class I dithiol glutaredoxins from poplar: biochemical basis for the definition of two subclasses. Frontiers in Plant Science 4:518. doi: 10.3389/fpls.2013.00518.

P22. Gao H, Subramanian S, Couturier J, Naik SG, Kim SK, Leustek T, Knaff DB, Wu HC, Vignols F, Huynh BH, Rouhier N, Johnson MK (2013) Arabidopsis thaliana Nfu2 accommodates [2Fe-2S] or [4Fe-4S] clusters and is competent for in vitro maturation of chloroplast [2Fe-2S] and [4Fe-4S] cluster-containing proteins. Biochemistry 52:6633-6645. doi: 10.1021/bi4007622.

P21. Zhang B, Bandyopadhyay S, Shakamuri P, Naik SG, Huynh BH, Couturier J, Rouhier N, Johnson MK (2013) Monothiol glutaredoxins can bind linear [Fe3S4]+ and [Fe4S4]2+ clusters in addition to [Fe2S2]2+ clusters: spectroscopic characterization and functional implications. J Am Chem Soc. 135:15153-15164. doi: 10.1021/ja407059n.

P20. Couturier J, Touraine B, Briat JF, Gaymard F, Rouhier N (2013) The iron-sulfur cluster assembly machineries in plants: current knowledge and open questions. Frontiers in Plant Science 4:259. doi: 10.3389/fpls.2013.00259.

P19. Couturier J, Chibani K, Jacquot JP, Rouhier N (2013) Cysteine-based redox regulation and signaling in plants. Frontiers in Plant Science 4:105. doi: 10.3389/fpls.2013.00105.

P18. Mapolelo D, Zhang B, Randeniya S, Albetel AN, Li H, Couturier J, Rouhier N, Outten CE, Johnson MK (2013) Monothiol glutaredoxins and A-type proteins: Partners in Fe-S cluster trafficking. Dalton Transactions 42:3107-3115. doi: 10.1039/c2dt32263c.

P17. Couturier J, Prosper P, Winger AM, Hecker A, Hirasawa M, Knaff D, Gans P, Jacquot JP, Navaza A, Haouz A, Rouhier N (2013) In the absence of thioredoxins, what are the reductants for peroxiredoxins in Thermotoga maritima? Antioxidants and Redox Signaling 18:1613-1622. doi: 10.1089/ars.2012.4739.

P16. Benyamina SM, Baldacci-Cresp F, Couturier J, Chibani K, Hopkins J, Bekki A, de Lajudie P, Rouhier N, Jacquot JP, Alloing G, Puppo A, Frendo P (2013) Two Sinorhizobium meliloti glutaredoxins regulate iron metabolism and symbiotic bacteroid differentiation. Environmental Microbiology 15:795-810. doi: 10.1111/j.1462-2920.2012.02835.x.

 

2012

P15. Couturier J, Vignols F, Jacquot JP, Rouhier N (2012) Glutathione- and glutaredoxin-dependent reduction of methionine sulfoxide reductase A. FEBS Letters 586: 3894-3899. doi: 10.1016/j.febslet.2012.09.020.

P14. Bedhomme M, Adamo M, Marchand CH, Couturier J, Rouhier N, Lemaire SD, Zaffagnini M, Trost P (2012) Glutathionylation of cytosolic glyceraldehyde-3-phosphate dehydrogenase from the model plant Arabidopsis thaliana is reversed by both glutaredoxins and thioredoxins in vitro. Biochemical Journal. 445: 337-347. doi: 10.1042/BJ20120505.

P13. Zaffagnini M, Bedhomme M, Marchand CH, Couturier J, Gao XH, Rouhier N, Trost P, Lemaire SD (2012) Glutaredoxin S12: unique properties for redox signaling. Antioxidants and Redox Signaling 16: 17-32. doi: 10.1089/ars.2011.3933.

2011

P12. Couturier J, Ströher E, Albetel AN, Roret T, Muthuramalingam M, Tarrago L, Seidel T, Tsan P, Jacquot JP, Johnson MK, Dietz KJ, Didierjean C, Rouhier N (2011) Arabidopsis chloroplastic glutaredoxin C5 as a model to explore molecular determinants for iron-sulfur cluster binding into glutaredoxins. The Journal of Biological Chemistry 286: 27515-27527.

 

2010

P11. Couturier J, Didierjean C, Jacquot JP, Rouhier N (2010) Engineered mutated glutaredoxins mimicking peculiar plant class III glutaredoxins bind iron-sulfur centers and possess reductase activity. Biochemical and Biophysical Research Communications 403: 435-441.

P10. Couturier J, Doidy J, Guinet F, Wipf D, Blaudez D, Chalot M (2010) Glutamine, arginine and the amino acid transporter Pt-CAT11 play important roles during senescence in poplar. Annals of Botany 105: 1159-1169.

P9. Couturier J, de Faÿ E, Fitz M, Wipf D, Blaudez D, Chalot M (2010) PtAAP11, a high affinity amino acid transporter specifically expressed in differentiating xylem cells of poplar. Journal of Experimental Botany 61: 1671-1682.

P8. Rouhier N, Couturier J, Johnson MK, Jacquot JP (2010) Glutaredoxins: roles in iron homeostasis. Trends in Biochemical Sciences 35: 43-52.

P7. Chibani K, Couturier J, Selles B, Jacquot JP, Rouhier N (2010) The chloroplastic thiol reducing systems: dual functions in the regulation of carbohydrate metabolism and regeneration of antioxidant enzymes, emphasis on the poplar redoxin equipment. Photosynthesis Research 104: 75-99.

 

2009

P6. Selles B, Rouhier N, Chibani K, Couturier J, Gama F, Jacquot JP (2009) Glutaredoxin: The Missing Link Between Thiol-Disulfide Oxidoreductases and Iron Sulfur Enzymes. Advances in Botanical Research 52: 405-436

P5. Couturier J, Jacquot JP, Rouhier N (2009) Evolution and diversity of glutaredoxins in photosynthetic organisms. Cellular and Molecular Life Sciences 66: 2539-2557.

P4. Couturier J1, Koh CS1, Zaffagnini M1, Winger A, Gualberto JM, Corbier C, Decottignies P, Jacquot JP, Lemaire SD, Didierjean C, Rouhier N (2009) Structure-function relationship of the chloroplastic GrxS12 with an atypical WCSYS active site. The Journal of Biological Chemistry 284: 9299-9310.

 

2007

P3. Couturier J, Montanini B, Martin F, Brun A, Blaudez D, Chalot M (2007) The expanded family of ammonium transporters in the perennial poplar plant. New Phytologist 174:137-150.

 

2006

P2. Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, Dejardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasjarvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Leple JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouze P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D (2006) The genome of black cottonwood, Populus trichocarpa (Torr. & Gray). Science 313: 596-604.

P1. Rouhier N, Couturier J, Jacquot JP (2006) Genome-wide analysis of plant glutaredoxin systems. Journal of Experimental Botany 57: 1685-96.