Hecker Arnaud

Bio: I received a Ph.D in Biology, Cellular and Molecular Biology from the University of Burgundy (Dijon) in 2002. Under the supervision of Pr. Gilles Luquet, my research activities focused on the structure-function analysis of Orchestin, a protein involved in a biomineralization process in the crustacea Orchestia cavimana. Later, I was hired in 2003 as non-permanent lecturer at the University of Burgundy (Dijon). Under the supervision of Dr. Frédéric Marin, I was interested in the biochemical characterization of proteins involved in the shell formation of the mollusk Pina nobilis. Then, I moved in 2004 to the Institute of Genetics and Microbiology (University of Paris-Sud XI). In the team headed by Pr. Patrick Forterre, my research activities focused in Archaea on deciphering the role and functions of KEOPS complex, a highly conserved complex essential for the maturation of the universal t6A tRNA modification. Finally, I joined  in September 2010 the Stress Response and Redox regulation team as assistant professor.

Present research: My current work focuses on two main topics: the biochemical and structural analysis of glutathione transferases from poplar and the study of molecular determinants in the context of the interaction between the pathogenic fungus Melampsora larici and the tree Populus trichocarpa.


Peer-reviewed publications


Gütle, D.D., Roret, T., Hecker, A., Reski, R. and Jacquot,  J.P. (2017). Dithiol disulphide exchange in redox regulation of chloroplast enzymes in response to evolutionary and structural constraints. Plant Sci. 255, 1-11.


Schwartz, M., Didierjean, C., Hecker, A., Girardet, J.M., Morel-Rouhier, M., Gelhaye, E. and Favier, F. (2016). Crystal Structure of Saccharomyces cerevisiae ECM4, a Xi-class glutathione transferase that reacts with glutathionyl-(hydro)quinones. PLoS One 11, e0164678.

Gütle, D.D., Roret, T., Müller, S.J., Couturier, J., Lemaire, S.D., Hecker, A., Dhalleine, T., Buchanan, B.B., Reski, R., Einsle, O. and Jacquot J.P. (2016). Chloroplast FBPase and SBPase are thioredoxin-linked enzymes with similar architecture but different evolutionary histories. Proc. Natl. Acad. Sci. U.S.A. 113, 6779-6784.

Petre, B., Hecker, A., Germain, H., Tsan, P., Sklenar, J., Pelletier, G., Séguin, A., Duplessis, S. and Rouhier, N. (2016). The Poplar Rust-Induced Secreted Protein (RISP) Inhibits the growth of the leaf rust pathogen Melampsora laricipopulina and triggers cell culture alkalinisation. Front. Plant Sci. 7, 97.

Lallement, P.A., Roret, T., Tsan, P., Gualberto, J.M., Girardet, J.M., Didierjean, C., Rouhier, N. and Hecker, A. (2016). Insights into ascorbate regeneration in plants: investigating the redox and structural properties of dehydroascorbate reductases from Populus trichocarpa. Biochem J. 473, 717-731.

Jacquot, J.P., Couturier, J., Didierjean, C., Gelhaye, E., Morel-Rouhier, M., Hecker, A., Plomion, C., Gütle, D. and Rouhier, N. (2016). Structural and functional characterization of tree proteins involved in redox regulation: a new frontier in forest science. Ann. For. Sci. 73, 119–134.


Lorrain, C., Hecker, A. and Duplessis, S. (2015). Effector-Mining in the Poplar Rust Fungus Melampsora laricipopulina secretome. Front. Plant Sci. 6, 1051.

Deroy, A., Saiag, F., Kebbi-Benkeder, Z., Touahri, N., Hecker, A., Morel-Rouhier, M., Colin, F., Dumarcay, S., Gérardin, P. and Gelhaye, E. (2015). The GSTome reflects the chemical environment of white-rot fungi. PLoS One 10, e0137083.

Lallement, P.A., Meux, E., Gualberto, J.M., Dumarcay, S., Favier, F., Didierjean, C., Saul, F., Haouz, A., Morel-Rouhier, M., Gelhaye, E., Rouhier, N. and Hecker A. (2015). Glutathionyl-hydroquinone reductases from poplar are plastidial proteins that deglutathionylate both reduced and oxidized glutathionylated quinones. FEBS Lett. 589, 37-44.


Pégeot, H., Koh, C.S., Petre, B., Mathiot, S., Duplessis, S., Hecker, A., Didierjean, C. and Rouhier, N. (2014). The poplar Phi class glutathione transferase: expression, activity and structure of GSTF1. Front. Plant Sci. 5:712.

Lallement, P.A., Brouwer, B., Keech, O., Hecker, A. and Rouhier, N. (2014). The still mysterious roles of cysteine-containing glutathione transferases in plants. Front. Pharmacol. 5, 192.

Thiaville, P.C., El Yacoubi, B., Perrochia, L., Hecker, A., Prigent, M., Thiaville, J.J., Forterre, P., Namy, O., Basta, T. and de Crécy-Lagard V. (2014). Cross kingdom functional conservation of the core universally conserved threonylcarbamoyladenosine tRNA synthesis enzymes. Eukaryot. Cell 13, 1222-1231.

Lallement, P.A., Meux, E., Gualberto, J.M., Prosper, P., Didierjean, C., Saul, F., Haouz, A., Rouhier, N. and Hecker, A. (2014). Structural and enzymatic insights into Lambda glutathione transferases from Populus trichocarpa, monomeric enzymes constituting an early divergent class specific to terrestrial plants. Biochem. J. 462, 39-52.


Perrochia, L., Guetta, D., Hecker, A., Forterre, P. and Basta, T. (2013). Functional assignment of KEOPS/EKC complex subunits in the biosynthesis of the universal t6A tRNA modification. Nucleic Acids Res. 41, 9484-9499.

Perrochia, L., Crozat, E., Hecker, A., Zhang, W., Bareille, J., Collinet, B., van Tilbeurgh, H., Forterre, P. and Basta, T. (2013). In vitro biosynthesis of a universal t6A tRNA modification in Archaea and Eukarya. Nucleic Acids Res. 41, 1953-1964.

Couturier, J., Prosper, P., Winger, A.M., Hecker, A., Hirasawa, M., Knaff, D., Gans, P., Jacquot, J.P., Navaza, A., Haouz, A. and Rouhier, N. (2013). In the absence of thioredoxins, what are the reductants for peroxiredoxins in Thermotoga maritima? Antioxid. Redox Signal. 18, 1613-1622.


Hacquard, S., Petre, B., Frey, P., Hecker, A., Rouhier, N. and Duplessis, S. (2011). The poplar-poplar rust interaction: insights from genomics and transcriptomics. J. Pathog. 2011, 2011-716041.


Oberto, J., Breuil, N., Hecker, A., Farina, F., Brochier-Armanet, C., Culetto, E. and Forterre, P. (2009). Qri7/OSGEPL, the mitochondrial version of the universal Kae1/YgjD protein, is essential for mitochondrial genome maintenance. Nucleic Acids Res. 37, 5343-5352.

Muller, S., Urban, A., Hecker, A., Leclerc, F., Branlant, C. and Motorin, Y. (2009). Deficiency of the tRNATyr:Ψ35-synthase aPus7 in Archaea of the Sulfolobales order might be rescued by the H/ACA sRNA-guided Machinery. Nucleic Acids Res. 37, 1308-1322.

Hecker, A., Graille, M., Madec, E., Gadelle, D., Le Cam, E., van Tilbeurgh, H. and Forterre, P. (2009). The universal Kae1 protein and the associated Bud32 kinase (PRPK), a mysterious protein couple probably essential for genome maintenance in Archaea and Eukarya. Biochem. Soc. Trans. 37, 29-35.


Hecker, A., Lopreiato, R., Graille, M., Collinet, B., Forterre, P., Libri, D. and van Tilbeurgh, H. (2008). Structure of the archaeal Kae1/Bud32 fusion protein MJ1130: a model for the eukaryotic EKC/KEOPS subcomplex. EMBO J. 27, 2340–2351.


Hecker, A., Leulliot, N., Gadelle, D., Graille, M., Justome, A., Dorlet, P., Brochier, C., Quevillon-Cheruel, S., Le Cam, E., van Tilbeurgh, H. and Forterre, P. (2007). An archaeal orthologue of the universal protein Kae1 is an iron metalloprotein which exhibits atypical DNA-binding properties and apurinic-endonuclease activity in vitro. Nucleic Acids Res. 35, 6042-6051.

Gras, S., Chaumont, V., Fernandez, B., Carpentier, P., Charrier-Savournin, F., Schmitt, S., Pineau, C., Flament, D., Hecker, A., Forterre, P., Armengaud, J. and Housset, D. (2007). Structural insights into a new homodimeric self-activated GTPase family. EMBO Rep. 8, 569-575.


Marin, F., Amons, R., Stigter, M., Hecker, A., Luquet, G., Layrolle, P., Vicente, N., de Groot, K. and Westbroek, P. (2005). Caspartin and calprismin, two new proteins of the shell calcitic prisms of the Mediterranean fan mussel Pinna nobilis. J. Biol. Chem. 280, 33895-33908.


Hecker, A., Quennedey, B., Testenière, O., Quennedey, A., Graf, F. and Luquet, G. (2004). Orchestin, a calcium-binding phosphoprotein is a matrix component of two successive transitory calcified biomineralizations elaborated cyclically by a terrestrial crustacean. J. Struct. Biol. 146, 310-324.


Hecker, A., Testenière, O., Marin, F. and Luquet, G. (2003). Phosphorylation of serine residues is fundamental for the calcium-binding ability of Orchestin, a soluble matrix protein from crustacean calcium storage structures. FEBS Lett. 535, 49-54.


Raz, S., Testenière, O., Hecker, A., Weiner, S. and Luquet, G. (2002). Stable amorphous calcium carbonate is the main component of the calcium storage structures of a terrestrial crustacean. Biol. Bull., 203, 269-274.

Testenière, O., Hecker, A., Le Gurun, S., Quennedey, B., Graf, F. and Luquet, G. (2002). Characterization and spatio-temporal expression of Orchestin, a gene encoding an ecdysone-inducible calcium binding protein from a crustacean organic matrix. Biochem. J. 361, 327-335.

Book chapters

Jacquot, J.P., Dietz, K.J., Rouhier, N., Meux, E., Lallement, P.A., Selles, B. and Hecker, A. (2013). Redox Regulation in Plants: Glutathione and “Redoxin” Related Families. In Oxidative Stress and Redox Regulation, Jakob U. (Ed.), Springer, 213-231.

Luquet, G., Hecker, A. and Marin, F. (2004). Orchestin, a key calcium-binding phosphoprotein in the cyclic elaboration of two different ACC storage structures by the crustacean, Orchestia cavimana. Proceedings of the 8th ICCBMT, October 17-22, Banff Centre, Alberta, Canada, Landis W.L. & Sodek J. Eds., pp. 111-114.