Rouhier N.

Career and scientific interests

After a PhD in plant physiology, molecular biology and biochemistry, I have been recruited in 2004 as an assistant professor at the Université of Nancy, before obtaining a professor position in 2011 at the newly created Université of Lorraine. My research activities concern the understanding (i) of the role of the antioxidant and detoxifying systems in the physiology of poplar under environmental constraints and (ii) of the role of glutaredoxins in the regulation of iron metabolism. Hence, during the last decade, I focused an important part of my work on the structure-function relationship of antioxidant enzymes belonging to the thioredoxin, glutaredoxin, thiol peroxidase and methionine sulfoxide reductase (MSR) and glutathione transferase protein families, providing many mechanistic details for their catalytic cycle and mode of interactions. In particular, we have established the first 3D structures of a plant MSR and of a plant glutathione peroxidase and dissected the catalytic and Trx-mediated regeneration mechanisms involving three and two cysteine residues respectively. Both structures indicated that an ample conformational rearrangement occurred during the transition from the reduced to the oxidized state (coll Dr Claude Didierjean, Nancy University). Besides, we have determined that a MsrB with a single catalytic cysteine is preferentially reduced by Grxs, an unknown function of Grxs at that time (coll Dr Pascal Rey, CEA Cadarache). The current work is now centered on the structure-function analysis of specific classes of glutathione transferases, focusing in particular in the isolation and identification of physiological substrates explaining their implication in biotic or abiotic stress responses.

Regarding my second major project, we have previously demonstrated that several plant Grxs can assemble [2Fe-2S] centers in a homodimer. Using mutational and structural analyses, we have determined that the cluster is linked with the sulfur atoms of the two usually catalytic cysteines and two external glutathione molecules. This was first shown for GrxC1 (CGYC active site, class I) and then also for GrxS14 and S16 (CGFS active sites, class II). Using spectroscopic and biochemical tools, we have further demonstrated that these CGFS containing Grxs (S14 and S16) which contain a much more labile [2Fe-2S] center than GrxC1, were able to transfer it to potential physiological acceptor proteins as an apoferredoxin (coll Pr Michael Johnson, University of Georgia). These results which were consistent with some genetic information obtained in yeast, led us to propose a mechanism in which chloroplastic Grxs could constitute components of the plastidial assembly machinery cooperating with the SUF machinery to integrate Fe-S centres into recipient proteins. I am currently investigating more precisely the roles of these glutaredoxins and of their partners in the maturation of Fe-S clusters and in the regulation of iron homeostasis (coll. Dr Frédéric Gaymard, Florence Vignols, Pascal Rey, Janneke Balk & Olivier Keech).



– 2018-2020: Projet ANR 2017: MOBIFER: Dynamics of coumarin secretion by plant roots into the soil in the context of iron nutrition (coordinator C. Dubos)

– 2014-2017: Projet ANR 2013: Fe-S traffic: The cellular trafficking of iron-sulfur (Fe-S) clusters in plants (coordinator N. Rouhier)

– 2011-2013: Projet ANR 2010 Blan 1616 01: FIRES: Functions of glutaredoxins in Iron and REdox sensing and Signaling in plants. (coordinator N. Rouhier)

– 2009-2011: Projet INRA/FORMAS: The role of organellar glutaredoxins in iron-sulfur cluster biogenesis in plants: an interactomic study for the identification of protein partners (G. Wingsle (Umea Plant Science center, sweden)/ coordinator N. Rouhier)

– 2008-2010: Projet ANR Jeune Chercheur JC07_204825: The role of organellar CGFS containing glutaredoxins in iron-sulfur clusters assembly and biogenesis in plants. (coordinator N. Rouhier)

– 2006-2008: Projet ANR, Réseau de Génomique végétale-Genoplante 2010: GNP05010P: Repair mechanisms of oxidized proteins in chloroplasts of Arabidopsis thaliana. (coordinator Pascal Rey)


Scientific publications


Przybyla-Toscano J, Roland M, Gaymard F, Couturier J, Rouhier N. Roles and maturation of iron-sulfur proteins in plastids. J Biol Inorg Chem. 2018, in press

Gao H, Azam T, Randeniya S, Couturier J, Rouhier N, Johnson MK. Characterization and Maturation of the Fe-S Center in Arabidopsis thaliana Dihydroxyacid Dehydratase. J Biol Chem 2017, in press

Chibani K, Saul F, Didierjean C, Rouhier N, Haouz A. Structural snapshots along the reaction mechanism of the atypical poplar thioredoxin-like2.1. Febs Lett. 2018, in press


Peleh V, Zannini F, Backes S, Rouhier N*, Herrmann JM*. Erv1 of Arabidopsis thaliana can directly oxidize mitochondrial intermembrane space proteins in the absence of redox-active Mia40. BMC Biology. 2017 15(1):106

Przybyla-Toscano J, Roret T, Couturier J, Rouhier N. Roles of class II glutaredoxins in the maturation of Fe-S proteins. Metalloprotein Active Site Assembly in Encyclopedia of Inorganic and bioinorganic chemistry Wiley & Sons.

Zannini F, Couturier J, Keech O, Rouhier N. In vitro alkylation methods for assessing the protein redox state. Methods Mol. Biol. 2017, 1653:51-64.

Pégeot H, Mathiot S, Perrot T, Gense F, Hecker A, Didierjean C, Rouhier N. Structural plasticity among glutathione transferase Phi members: natural combination of catalytic residues confers dual biochemical activities. FEBS J. 2017, 284:2442-2463.

Rey P, Becuwe N, Tourrette S, Rouhier N. Involvement of Arabidopsis glutaredoxin S14 in the maintenance of chlorophyll content. Plant Cell Environ. 2017, 40:2319-2332.

Keech O, Gardeström P, Kleczkowski LA, Rouhier N. The redox control of photorespiration: from biochemical and physiological aspects to biotechnological considerations. Plant Cell Environ. 2017, 40(4):553-569.

Shaikhali J, Rouhier N, Hecker A, Brännström K, Wingsle G. Covalent and non-covalent associations mediate MED28 homo-oligomerization. Journal of Plant Biochemistry & Physiology. 2017, 5:189.

Selles B, Zannini F, Couturier J, Jacquot JP, Rouhier N. Atypical protein disulfide isomerases (PDI): Comparison of the molecular and catalytic properties of poplar PDI-A and PDI-M with PDI-L1A. PLoS One. 2017, 12:e0174753.

Castella C, Mirtziou I, Seassau A, Boscari A, Montrichard F, Papadopoulou K, Rouhier N, Puppo A, Brouquisse R. Post-translational modifications of Medicago truncatula glutathione peroxidase 1 induced by nitric oxide. Nitric Oxide. 2017, 68:125-136.

Werner Ribeiro C, Baldacci-Cresp F, Pierre O, Larousse M, Benyamina S, Lambert A, Hopkins J, Castella C, Cazareth J, Alloing G, Boncompagni E, Couturier J, Mergaert P, Gamas P, Rouhier N, Montrichard F, Frendo P. Regulation of Differentiation of Nitrogen-Fixing Bacteria by Microsymbiont-Targeting of Plant Thioredoxin s1. Current Biology. 2017, 27(2):250-256.


Jacquot JP, Couturier J, Didierjean C, Gelhaye E, Morel-Rouhier M, Hecker A, Plomion C, Gütle D, Rouhier N. Structural and Functional Characterization of Tree Proteins Involved in Redox Regulation: A new Frontier in Forest Science. Annals of Forest sciences. 2016, 1-16.

Lallement PA, Roret T, Tsan P, Gualberto JM, Girardet JM, Didierjean C, Rouhier N, Hecker A. Insights into ascorbate regeneration in plants: investigating the redox and structural properties of dehydroascorbate reductases from Populus trichocarpa. Biochem J. 2016, 473(6):717-31.

Petre B, Hecker A, Germain H, Tsan P, Sklenar J, Pelletier G, Séguin A, Duplessis S, Rouhier N. The Poplar Rust-Induced Secreted Protein (RISP) Inhibits the Growth of the Leaf Rust Pathogen Melampsora larici-populina and Triggers Cell Culture Alkalinisation. Front Plant Sci. 2016, 7:97.

Cardi M, Zaffagnini M, De Lillo A, Castiglia D, Chibani K, Gualberto JM, Rouhier N, Jacquot JP, Esposito S. Plastidic P2 glucose-6P dehydrogenase from poplar is modulated by thioredoxin m-type: Distinct roles of cysteine residues in redox regulation and NADPH inhibition. Plant Sci. 2016, 252:257-266.

Chrobok D, Law SR, Brouwer B, Lindén P, Ziolkowska A, Liebsch D, Narsai R, Szal B, Moritz T, Rouhier N, Whelan J, Gardestrom P, Keech O. Dissecting the metabolic role of mitochondria during developmental leaf senescence. 2016, Plant Physiol. 172:2132-2153.


Moseler A, Aller I, Wagner S, Nietzel T, Przybyla-Toscano J, Mühlenhoff U, Lill R, Berndt C, Rouhier N, Schwarzländer M, Meyer AJ. The mitochondrial monothiol glutaredoxin S15 is essential for iron-sulfur protein maturation in Arabidopsis thaliana. 2015 Proc Natl Acad Sci USA 2015, 112(44):13735-40.

Valiauga B, Rouhier N, Jacquot JP, Čėnas N. Quinone- and nitroreductase reactions of Thermotoga maritima thioredoxin reductase. Acta Biochima Polonica. 2015, 62(2):303-9.

Shaikhali J, Davoine C, Brännström K, Rouhier N, Bygdell J, Björklund S, Wingsle G. Biochemical and redox characterization of the Mediator complex and its associated transcription factor GeBPL, a GLABROUS1 enhancer binding protein. Biochem J. 2015, 468(3):385-400.

Matamoros MA, Saiz A, Peñuelas M, Bustos-Sanmamed P, Mulet JM, Barja MV, Rouhier N, Moore M, James EK, Dietz KJ, Becana M. Function of glutathione peroxidases in legume root nodules. J Exp Bot. 2015, 66(10):2979-90.

Knuesting J, Riondet C, Maria C, Kruse I, Bécuwe N, König N, Berndt C, Tourrette S, Guilleminot-Montoya J, Herrero E, Gaymard F, Balk J, Belli G, Scheibe R, Reichheld JP, Rouhier N, Rey P.. Glutaredoxin S17 and its partner NF-YC11/NC2α contribute to maintenance of the shoot apical meristem in Arabidopsis under long-day photoperiod. Plant Physiol. 2015, 167(4):1643-58.

Rouhier N, Cerveau D, Couturier J, Reichheld JP, Rey P. Involvement of thiol-based mechanisms in plant development. Biochim. Biophys. Acta. General Subject 2015, 1850(8):1479-1496.

Couturier J, Przybyla-Toscano J, Roret T, Didierjean C, Rouhier N. The roles of glutaredoxins integrating Fe-S clusters: sensing, transfer or repair functions. Biochim. Biophys. Acta. Mol cell res. 2015, 1853(6):1513-1527.

Lallement PA, Meux E, Gualberto JM, Dumarcay S, Favier F, Didierjean C, Saul F, Haouz A, Morel-Rouhier M, Gelhaye E, Rouhier N, Hecker A. Glutathionyl-hydroquinone reductases from poplar are plastidial proteins which deglutathionylate glutathione-conjugated reduced and oxidized quinones. Febs Lett. 2015, 589(1):37-44.


Pégeot H, Koh CS, Petre B, Mathiot S, Duplessis S, Hecker A, Didierjean C, Rouhier N. The poplar phi class glutathione transferase: expression, activity and structure of GSTF1. Front Plant Sci. 2014, 23;5:712.

Lallement PA, Brouwer B, Keech O, Hecker A, Rouhier N. The still mysterious roles of cysteine-containing glutathione transferases in plants. Frontiers in pharmacology. 2014, 20;5:192.

Jacquot JP, Couturier J, Didierjean C, Gelhaye E, Morel-Rouhier M, Hecker A, Plomion C, Gütle D, Rouhier N. Structural and Functional Characterization of Tree Proteins Involved in Redox Regulation: A new Frontier in Forest Science. Annals of Forest sciences. 2014, 1-16.

Roret T, Tsan P, Couturier J, Zhang B, Johnson MK, Rouhier N, Didierjean C. Structural and spectroscopic insights into BolA-glutaredoxin complexes. J Biol Chem 2014, 289(35):24588-98.

Roret T, Pégeot H, Couturier J, Mulliert G, Rouhier N, Didierjean C. X-ray structures of Nfs2, the plastidial cysteine desulfurase from Arabidopsis thaliana. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2014, 70(Pt 9):1180-5.

Petre B, Hacquard S, Duplessis S, Rouhier N. Genome analysis of poplar LRR-RLP gene clusters reveals RISP, a defense-related gene coding a candidate endogenous peptide elicitor. Front Plant Sci. 2014, 5:111.

Lallement PA, Meux E, Gualberto JM, Prosper P, Didierjean C, Saul F, Haouz A, Rouhier N, Hecker A. Structural and enzymatic insights into Lambda glutathione transferases from Populus trichocarpa, monomeric enzymes constituting an early divergent class specific to terrestrial plants. Biochem J. 2014, 462(1):39-52.

Dhalleine T, Rouhier N, Couturier J. Putative roles of glutaredoxin-BolA holo-heterodimers in plants. Plant Signal Behav. 2014, 9(3).

Wang L, Li Y, Jacquot JP, Rouhier N, Xia B. Characterization of poplar GrxS14 in different structural forms. Protein Cell 2014, 5(5):329-33.

Couturier J, Wu HC, Dhalleine T, Pégeot H, Sudre D, Gualberto JM, Jacquot JP, Gaymard F, Vignols F, Rouhier N. Monothiol glutaredoxin-BolA interactions: redox control of Arabidopsis thaliana BolA2 and SufE1. Mol Plant 2014, 7(1):187-205.

Cejudo FJ, Meyer AJ, Reichheld JP, Rouhier N, Traverso JA. Thiol-based redox homeostasis and signaling. Front. Plant Sci 2014, 5:266.


Aller I, Rouhier N, Meyer A. Development of roGFP2-derived redox probes for measurement of the glutathione redox potential in the cytosol of severely glutathione deficient rml1 seedlings. Front Plant Sci. 2013, 16;4:506.

Couturier J, Jacquot JP, Rouhier N. Towards a refined classification of class I dithiol glutaredoxins from poplar: biochemical basis for the definition of two subclasses. Front Plant Sci. 2013, 18;4:518.

Cardi M, Chibani K, Castiglia D, Cafasso D, Pizzo E, Rouhier N, Jacquot JP, Esposito S. Overexpression, purification and enzymatic characterization of a recombinant plastidial glucose-6-phosphate dehydrogenase from barley (Hordeum vulgare cv. Nure) roots. Plant Physiol Biochem, 2013, 17;73C:266-273.

Zhang B, Bandyopadhyay S, Shakamuri P, Naik SG, Huynh BH, Couturier J, Rouhier N, Johnson MK. Monothiol Glutaredoxins Can Bind Linear [Fe3S4]+ and [Fe4S4]2+ Clusters in Addition to [Fe2S2]2+ Clusters: Spectroscopic Characterization and Functional Implications. J Am Chem Soc. 2013, 135(40):15153-64.

Gao H, Subramanian S, Couturier J, Naik SG, Kim SK, Leustek T, Knaff DB, Wu HC, Vignols F, Huynh BH, Rouhier N, Johnson MK. Arabidopsis thaliana Nfu2 Accommodates [2Fe-2S] or [4Fe-4S] Clusters and Is Competent for in Vitro Maturation of Chloroplast [2Fe-2S] and [4Fe-4S] Cluster-Containing Proteins. Biochemistry. 2013, 52(38):6633-45.

Couturier J, Touraine B, Briat JF, Gaymard F, Rouhier N. The iron-sulfur cluster assembly machineries in plants: current knowledge and open questions. Front Plant Sci. 2013, 24;4:259.

Zaffagnini M, Morisse S, Bedhomme M, Marchand CH, Festa M, Rouhier N, Lemaire SD, Trost P. Mechanisms of nitrosylation and denitrosylation of cytoplasmic glyceraldehyde-3-phosphate dehydrogenase from Arabidopsis thaliana. J Biol Chem. 2013, 288(31):22777-89.

Couturier J, Chibani K, Jacquot JP, Rouhier N. Cysteine-based redox regulation and signalling in plants. Front Plant Sci. 2013, 29;4:105.

Jacquot JP, Dietz KJ, Rouhier N, Meux E, Lallement PA, Selles B, and Hecker A. Redox regulation in plants: Glutathione and “Redoxin” related families. Springer Book Oxidative stress and redox regulation 2013

Messens J, Rouhier N, Collet JF. Redox Homeostasis. Springer Book Oxidative stress and redox regulation 2013

Benyamina SM, Baldacci-Cresp F, Couturier J, Chibani K, Hopkins J, Bekki A, de Lajudie P, Rouhier N, Jacquot JP, Alloing G, Puppo A, Frendo P. Two Sinorhizobium meliloti glutaredoxins regulate iron metabolism and symbiotic bacteroid differentiation. Env. Microbiol. 2013, 15(3):795-810

Mapolelo DT, Zhang B, Randeniya S, Albetel AN, Li H, Outten CE, Couturier J, Rouhier N, Johnson MK. Monothiol glutaredoxins and A-type proteins: Partners in Fe-S cluster trafficking. Dalt trans. 2013 42(9):3107-15.

Couturier J, Prosper P, Winger AM, Hecker A, Hirasawa M, Knaff DB, Gans P, Jacquot JP, Navaza A, Haouz A, Rouhier N. In the absence of thioredoxins, what are the reductants for peroxiredoxins in Thermotoga maritima? Antioxid Redox Signal. 2013, 18(13):1613-22



Zaffagnini M, Bedhomme M, Marchand CH, Couturier J, Gao XH, Rouhier N, Trost P, Lemaire SD. Glutaredoxin S12: unique properties for redox signaling. Antioxid Redox Signal. 2012, 16:17-32.

Tarrago L, Kieffer-Jaquinod S, Lamant T, Marcellin MN, Garin JR, Rouhier N, Rey P. Affinity chromatography: a valuable strategy to isolate substrates of methionine sulfoxide reductases? Antioxid Redox Signal. 2012, 16:79-84.

Selles B, Hugo M, Trujillo M, Srivastava V, Wingsle G, Jacquot JP, Radi R, Rouhier N. Hydroperoxide and peroxynitrite reductase activity of poplar thioredoxin-dependent glutathione peroxidase 5 : kinetics, catalytic mechanism and oxidative inactivation. Biochem J. 2012, 442(2):369-80.

Chibani K, Tarrago L, Gualberto JM, Wingsle G, Rey P, Jacquot JP, Rouhier N. Atypical thioredoxins in poplar: the glutathione-dependent thioredoxin-like 2.1 supports the activity of target enzymes possessing a single redox active cysteine. Plant Physiol. 2012, 159(2):592-605.

Bedhomme M, Adamo M, Marchand CH, Couturier J, Rouhier N, Lemaire SD, Zaffagnini M, Trost P. Glutathionylation of cytosolic glyceraldehyde-3-phosphate dehydrogenase from the model plant Arabidopsis thaliana is reversed by both glutaredoxins and thioredoxins in vitro. Biochem J. 2012, 445(3):337-47.

Petre B, Morin E, Tisserant E, Hacquard S, Da Silva C, Poulain J, Delaruelle C, Rouhier N, Martin F, Kohler A, Duplessis S. RNA-Seq of early-infected poplar leaves by the rust pathogen Melampsora laricipopulina uncovers PtSultr3;5, a fungal-induced host sulfate transporter. Plos one, 2012;7(8):e44408.

Wang L, Ouyang B, Li Y, Feng Y, Jacquot JP, Rouhier N, Xia B. Glutathione regulates the transfer of iron-sulfur cluster from monothiol and dithiol glutaredoxins to apo ferredoxin. Protein Cell, 2012, 3(9):714-21.

Anusevičius T, Misevičiene L, Šarlauskas J, Rouhier N, Jacquot JP, Čėnas N. Quinone- and nitroreductase reactions of Thermotoga maritime peroxiredoxin-nitroreductase hybrid enzyme. Arch Biochem Biophys, 2012, 528(1):50-6.

Couturier J, Vignols F, Jacquot JP, Rouhier N. Glutathione- and glutaredoxin-dependent reduction of methionine sulfoxide reductase A. Febs letters, 2012, 586(21):3894-9.



Wang L, Ren X, Li Y, Rouhier N, Jacquot JP, Jin C, Xia B. (1)H, (13)C, and (15)N resonance assignments of reduced GrxS14 from Populus tremula × tremuloides. Biomol NMR Assign. 2011 5(1):121-4.

Selles B, Jacquot JP, Rouhier N. Comparative genomic study of protein disulfide isomerases from photosynthetic organisms. Genomics. 2011, 97(1):37-50.

Bunet R, Song L, Mendes MV, Corre C, Hotel L, Rouhier N, Framboisier X, Leblond P, Challis GL and Aigle B. Characterization and manipulation of the pathway-specific late regulator AlpW reveals Streptomyces ambofaciens as a new producer of kinamycins. J Bacteriol. 2011, 193(5):1142-53

Petre B, Major I, Rouhier N, Duplessis S. Genome-wide analysis of eukaryote thaumatin-like proteins (TLPs) with an emphasis on poplar. BMC Plant Biol. 2011, 15:11:33.

Cardi M, Chibani K, Cafasso D, Rouhier N, Jacquot JP, Esposito S. Abscisic acid effects on activity and expression of barley (Hordeum vulgare) plastidial glucose-6-phosphate dehydrogenases. J Exp Bot. 2011, 62:4013-23.

Duplessis S, Cuomo CA, Lin YC, Aerts A, Tisserant E, Veneault-Fourrey C, Joly DL, Hacquard S, Amselem J, Cantarel BL, Chiu R, Couthinho P, Feau N, Field M, Frey P, Gelhaye E, Goldberg J, Grabherr M, Kodira CD, Kohler A, Kües U, Lindquist EA, Lucas S, Mago R, Mauceli E, Morin E, Murat C, Pangilinan JL, Park R, Pearson M, Quesneville H, Rouhier N, Sakthikumar S, Salamov AA, Schmutz J, Selles B, Shapiro H, Tangay P, Tuskan GA, Henrissat B, Van de Peer Y, Rouzé P, Ellis JG, Dodds PN, Schein JE, Zhong S, Hamelin RC, Grigoriev IV, Szabon LJ, Martin F. Obligate Biotrophy Features Unraveled by the Genomic Analysis of Rust Fungi. Proc Natl Acad Sci USA 2011, 108(22):9166-71

Chibani K, Tarrago L, Schürmann P, Jacquot JP, Rouhier N. Biochemical properties of poplar thioredoxin z. FEBS Lett. 2011, 585:1077-81.

Méndez A, Matamoros MA, Bustos-Sanmamed P, Dietz KJ, Cejudo FJ, Rouhier N, Sato S, Tabata S, Becana M. Peroxiredoxins and NADPH-Dependent Thioredoxin Systems in the Model Legume Lotus japonicus. Plant Physiol. 2011, 156:1535-47.

Couturier J, Stroher E, Albetel AN, Roret T, Muthuramalingam M, Tarrago L, Seidel T, Tsan P, Jacquot JP, Johnson MK, Dietz KJ, Didierjean C, Rouhier N. Arabidopsis chloroplastic glutaredoxin C5 as a model to explore the molecular determinants for iron-sulfur cluster binding into glutaredoxins. J Biol Chem. 2011, 286:27515-27.

Rouhier N, Jacquot JP, Misevčiene L, Anusevičius Z, Šarlauskas J, Čėnas N. Two-electron reduction of nitroaromatic compounds by Thermotoga maritima hybrid peroxiredoxin-nitroreductase enzyme. Chemija 2011, 22 :143-148.

Hacquard S, Petre B, Frey P, Hecker A, Rouhier N, Duplessis S. The poplar-poplar rust interaction: insights from genomics and transcriptomics. J. Pathogens 2011;2011:716041.



Couturier, J., Didierjean, C., Jacquot, J.-P., Rouhier, N. Engineered mutated glutaredoxins mimicking peculiar plant class III glutaredoxins bind iron-sulfur centers and possess reductase activity. Biochem Biophys Res Comm 2010, 403(3-4):435-41.

Rouhier N, Couturier J, Johnson MK, Jacquot JP. Glutaredoxins: roles in iron homeostasis. Trends Biochem Sci. 2010, 35(1):43-52.

Chibani K, Couturier J, Selles B, Jacquot JP, Rouhier N. The chloroplastic thiol reducing systems: dual functions in the regulation of carbohydrate metabolism and regeneration of antioxidant enzymes, emphasis on the poplar redoxin equipment. Photosynth Res. 2010, 104(1):75-99.

Rouhier N. Plant glutaredoxins: pivotal players in redox biology and iron-sulphur centre assembly. New Phytol 2010, 186(2):365-72.

Jacquot JP, Rouhier N. Introduction. Photosynth Res. 2010, 104(1):1-3.



Ramos J, Matamoros M, Nayal L, James EK, Rouhier N, Sato S, Tabata S, Becana M. The glutathione peroxidase gene family of Lotus japonicus. Characterization of genomic clones, expression analyses and immunolocalization in legumes. New Phytol. 2009, 181(1):103-14.

Chibani K, Wingsle G, Jacquot JP, Gelhaye E, Rouhier N. Comparative genomic study of the thioredoxin family in photosynthetic organisms with emphasis on Populus trichocarpa. Mol. Plant. 2009, 2 :308-322

Couturier J, Koh CS, Zaffagnini M, Winger A, Gualberto JM, Corbier C, Decottignies P, Jacquot JP, Lemaire SD, Didierjean C, Rouhier N. Structure function relationship of the chloroplastic GrxS12 with an atypical WCSYS active site. J Biol Chem. 2009, 284(14):9299-310.

Srivastava V, Kumar Srivastava M, Chibani K, Nilsson R,Rouhier N, Melzer M, Wingsle G. Alternative Splicing Studies of the ROS Gene Network in Populus Reveal Two Isoforms of High Iso-electric Point SuperoxideDismutase. Plant physiol. 2009, 149(4):1848-59.

Tarrago L, Laugier E, Zaffagnini M, Marchand C, Le Maréchal P, Rouhier N, Lemaire SD, Rey P. Regeneration mechanisms of Arabidopsis thaliana methionine sulfoxide reductases B by glutaredoxins and thioredoxins.J Biol Chem. 2009, 284(28):18963-71.

Couturier J, Jacquot JP, Rouhier N. Evolution and diversity of glutaredoxins in photosynthetic organisms. Cell Mol Life Sci. 2009, 66(15):2539-57.

Jacquot JP, Eklund H, Rouhier N, Schürmann P. Structural and evolutionary aspects of thioredoxin reductases in photosynthetic organisms. Trends Plant Sci. 2009, 14(6):336-43.

Selles B, Rouhier N, Chibani K, Couturier J, Gama F, Jacquot JP. Chapter 13 Glutaredoxin. The Missing Link Between Thiol-Disulfide Oxidoreductases and Iron Sulfur Enzymes. 2009 Advances in Botanical Research 52 (C), pp. 405-436



Barbey C, Rouhier N, Haouz A, Navaza A, Jacquot JP. Expression, purification, crystallization and preliminary X-ray analysis of the peroxiredoxin domain of a larger natural hybrid protein from Thermotoga maritima. Acta Crystallogr Sect F Struct Biol Cryst Commun.  2008, 64:29-31.

Martin F, Aerts A, Ahrén D, Brun A, Danchin EG, Duchaussoy F, Gibon J, Kohler A, Lindquist E, Pereda V, Salamov A, Shapiro HJ, Wuyts J, Blaudez D, Buée M, Brokstein P, Canbäck B, Cohen D, Courty PE, Coutinho PM, Delaruelle C, Detter JC, Deveau A, DiFazio S, Duplessis S, Fraissinet-Tachet L, Lucic E, Frey-Klett P, Fourrey C, Feussner I, Gay G, Grimwood J, Hoegger PJ, Jain P, Kilaru S, Labbé J, Lin YC, Legué V, Le Tacon F, Marmeisse R, Melayah D, Montanini B, Muratet M, Nehls U, Niculita-Hirzel H, Oudot-Le Secq MP, Peter M, Quesneville H, Rajashekar B, Reich M, Rouhier N, Schmutz J, Yin T, Chalot M, Henrissat B, Kües U, Lucas S, Van de Peer Y, Podila GK, Polle A, Pukkila PJ, Richardson PM, Rouzé P, Sanders IR, Stajich JE, Tunlid A, Tuskan G, Grigoriev IV. The genome of Laccaria bicolor provides insights into mycorrhizal symbiosis. Nature. 2008, 452:88-92.

Bunet R, MendesMV, RouhierN, PangX, HotelL, DecarisB, LeblondP, AigleB. Regulation of the angucyclinone antibiotic alpomycin in Streptomyces ambofaciens by the autoregulator receptor AlpZ and its specific ligand. J. Bacteriol. 2008, 190:3293-305.

Gama F, Bréhelin C, Gelhaye E, Meyer Y, Jacquot JP, Rey P, Rouhier N. Biochemical and functional analysis of chloroplastic Prx IIE. Physiol Plant. 2008, 133:599-610.

Bandyopadhyay S, Gama F, Molina-Navarro MM, Gualberto JM, Claxton R, Naik SG, Huynh BH, Herrero E, Jacquot JP, Johnson MK, Rouhier N. Chloroplast monothiol glutaredoxins as scaffold proteins for the assembly and delivery of [2Fe-2S] clusters. EMBO J. 2008, 27:1122-33

Morel M, Kohler A, Martin F, Gelhaye E, Rouhier N. Comparison of the thiol-dependent antioxidant systems in the ectomycorrhizal Laccaria bicolor and the saprotrophic Phanerochaete chrysosporium. New Phytol. 2008, 180(2):391-407.

Koh CS, Navrot N, Didierjean C, Rouhier N, Hirasawa M, Knaff DB, Wingsle G, Samian R, Jacquot JP, Corbier C, Gelhaye E. An atypical catalytic mechanism involving three cysteines of thioredoxin. J Biol Chem. 2008, 283:23062-23072.

Rouhier N, Jacquot JP. Getting sick may help plants overcome abiotic stress. New Phytol. 2008, 180 : 738-741.

Rouhier N, Lemaire SD, Jacquot JP. The role of glutathione in photosynthetic organisms : emerging functions for glutaredoxin and glutathionylation. Annu. Rev. Plant Biol. 2008, 59:143-166.

Rouhier N, Koh CS, Gelhaye E, Corbier C, Tete-Favier F, Didierjean C, Jacquot JP. Redox based anti-oxidant systems in plants: biochemical and structural analyses. Biochim. Biophys. Acta. 2008, 1780:1249-60.



Gama F, Keech O, Eymery F, Finkemeier I, Gelhaye E, Gardeström P, Dietz KJ, Rey P, Jacquot JP, Rouhier N. The mitochondrial type II peroxiredoxin from poplar Physiol. Plant. 2007, 129(1) :196-206.

Rouhier N, Kauffmann B, Tete-Favier F, Palladino P, Gans P, Branlant G, Jacquot JP, Boschi-Muller S. Functional and structural aspects of poplar cytosolic and plastidial type a methionine sulfoxide reductases. J. Biol. Chem. 2007, 282(5):3367-78.

Rouhier N, Hideaki Unno H, BandyopadhyayS, Masip L,Kim SK, Hirasawa M, Gualberto JM, Lattard V, Kusunoki M, Knaff DB, Georgiou G, Hase T, Johnson MK, Jacquot JP. Functional, structural and spectroscopic characterization of a glutathione-ligated [2Fe-2S] cluster in poplar glutaredoxin C1. Proc Natl Acad Sci USA 2007, 104(18):7379-84.

Koh CS, Didierjean C, Navrot N,  Panjikar S, Mulliert G, Rouhier N, Jacquot JP, Aubry A, Shawkataly O, Corbier C. Crystal structure of a poplar thioredoxin peroixdase which exhibits the structure of glutathione peroxidases : insights into redox driven conformational changes. J. Mol. Biol. 2007, 370(3):512-29.

Passardi F, Zamocky M, Cosio C,  Rouhier N, Theiler G, Penel C, and Dunand C. PeroxiBase: the peroxidase database. Phytochemistry 2007, 68(12):1605-11.

Meyer AJ, Brach T, Marty L, Kreye S, Rouhier N, Jacquot JP, Hell R. Redox-sensitive GFP in Arabidopsis thaliana is a quantitative biosensor for the redox potential of the cellular glutathione redox buffer. Plant J. 2007, 52(5):973-86

Vieira Dos Santos C, Laugier E, Tarrago L, Massot V, Issakidis-Bourguet E, Rouhier N Rey P.Specificity of thioredoxins and glutaredoxins as electron donors to two distinct classes of Arabidopsis plastidial methionine sulfoxide reductases B FEBS Lett. 2007, 581(23):4371-6.

Navrot N, Rouhier N, Gelhaye E, Jacquot JP. ROS generation and antioxidant systems in plant mitochondria. Physiol. Plantarum 2007, 129(1):185-195.


Rouhier N, Gama F, Wingsle G, Gelhaye E, Gans P, Jacquot JP. Engineering artificial functional hybrid proteins between poplar peroxiredoxin II and glutaredoxin or thioredoxin. Biochem. Biophys. Res Comm. 2006, 341(4):1300-1308.

Feng Y,  Zhong N, Rouhier N, Hase T, Kusunoki K, Jacquot JP, Jin C, Xia B. Structural insight into poplar glutaredoxin C1 with a bridging iron sulfur cluster at the active site. Biochemistry. 2006, 45(26):7998-8008

Noguera-Mazon V, Lemoine J, Walker O, Rouhier N, Salvador A, Jacquot JP, Lancelin JM, Krimm I. Glutathionylation induces the dissociation of 1-Cys D-peroxiredoxin non-covalent homodimer. J Biol Chem. 2006, 281(42):31736-42.

Gelhaye E, Navrot N, Macdonald IK, Rouhier N, Lloyd Raven E, Jacquot JP. Ascorbate Peroxidase-Thioredoxin Interaction. Photosynth Res. 2006, 89(3):1-8.

Navrot N, Collin V, Gualberto J, Gelhaye E, Hirasawa M, Rey P, Knaff DB, Issakidis E, Jacquot JP, Rouhier N. Plant glutathione peroxidases are functional peroxiredoxins distributed in several subcellular compartments and regulated during biotic and abiotic stresses. Plant Physiol. 2006, 142(4):1364-79.

Rouhier N, Couturier J, Jacquot JP. Genome-wide analysis of plant glutaredoxin systems. J. Exp. Bot. 2006, 57(8):1685-96.

Navrot N, Gelhaye E, Jacquot JP, Rouhier N. Identification of a new family of plant proteins loosely related to glutaredoxins with four CxxC motives. Photosynth. Res. 2006. 89(2):71-9.

Rouhier N, Vieira Dos Santos C, Tarrago L Rey P. Plant methionine sulfoxide reductase A and B multigenic families. Photosynth Res. 2006, 89(3):1-16.


Rey P, Cuiné S, Eymery F, Garin J, Court M, Jacquot JP, Rouhier N, Broin M. Analysis of the proteins targeted by CDSP32, a plastidic thioredoxin participating in oxidative stress responses. Plant J, 2005, 41:31-42.

Echalier A, Trivelli X, Corbier C, Rouhier N, Walker O, Tsan P,  Jacquot JP, Krimm I, Lancelin JM. X-ray and NMR studies of a homodimeric D (Type II) peroxiredoxin reduced by both glutaredoxin and thioredoxin : Prx-Prx interfaces in the Prx family. Biochemistry, 2005, 44:1755-67.

Mabicka A, Dumarcay S, Rouhier N, Linder M, Jacquot JP, Gerardin P, Gelhaye E. Synergistic wood preservatives involving EDTA, inorganic 1076 and 2-hydroxypyridine-N-oxide. International biodeterioration and biodegradation. 2005, 55 (3), 203-211.

Feng Y, Rouhier N, Jacquot JP, Xia B. Letter to the Editor: (1)H, (15)N, and (13)C resonance assignments of reduced glutaredoxin C1 from Populus tremula x tremuloides. J Biomol NMR. 2005, 31:263-4.

Vieira Dos Santos C, Cuine S, Rouhier N, Rey P. The Arabidopsis plastidic methionine sulfoxide reductase B proteins. Sequence and activity characteristics, comparison of the expression with plastidic methionine sulfoxide reductase a, and induction by photooxidative stress. Plant Physiol. 2005, 138(2):909-22.

Rouhier N, Villarejo A, Srivastava M, Gelhaye E, Keech O, Droux M, Finkemeier I, Samuelsson G, Dietz KJ, Jacquot JP, WingsleG. Identification of plant glutaredoxin targets. Antiox Redox Signal. 2005, 7(7-8):919-929.

Noguera V, Walker O, Rouhier N, Jacquot JP, Krimm I, Lancelin JM. NMR reveals a novel glutaredoxin glutaredoxin interaction interface. J. Mol. Biol. 2005, 353(3):629-41.

Gelhaye E, RouhierN, Navrot N, Jacquot JP. The plant thioredoxin systems. Cell. Mol. Life Sci. 2005, 62:24-35.

Rouhier N, Jacquot JP. The plant multigenic family of thiol peroxidases. Free Radic. Biol. Med. 2005, 38 (11):1413-21.


Rouhier N, Gelhaye E, Corbier C Jacquot JP. Active site mutagenesis and phospholipid hydroperoxide reductase activity of poplar type II peroxiredoxin. Physiol. Plant. 2004, 120(1):57-62.

Gelhaye E, Rouhier N, Jacquot JP. Evidence for a subgroup of thioredoxin h that requires GSH/Grx for its reduction. FEBS Lett. 2004, 555(3): 443-448.

Rouhier N, Gelhaye E, Gualberto J, Jordy MN, De Fay E, Hirasawa M, Duplessis S, Lemaire S, Frey P, Martin F, Manieri W, Knaff DB, Jacquot JP. Poplar peroxiredoxin Q : a thioredoxin-linked chloroplast antioxidant functional in pathogen defense. Plant Physiol. 2004, 134(3):1027-38.

Balmer Y, Vensel WH, Tanaka CK, Hurkman WJ, Gelhaye E, RouhierN, JacquotJP, Manieri W, Schürmann P, Droux M, Buchanan BB. Thioredoxin links redox to the regulation of fundamental processes of plant mitochondria. Proc. Natl. Acad. Sci. USA 2004, 101(8):2642-7.

Jacquot JP, RouhierN, Navrot N, Decottignies JP. Generation of polyclonal antibodies against multiple proteins in a single rabbit and subsequent isolation of the specific immunoglobulins as a tool for proteomics research. Biotechnol. App. Bioc. 2004, 40(Pt 2):181-5.

Bouillac S, Rouhier N, Tsan P, Jacquot JP, Lancelin JM. 1H, (13)C and (15)N NMR assignment of the homodimeric poplar phloem type II peroxiredoxin. J Biomol NMR. 2004 Sep;30(1):105-6.

Noguera V, Rouhier N, Krimm I, Jacquot JP, Lancelin JM. 1H, 13C and 15N NMR assigment of the homodimeric poplar phloem peroxiredoxin. J Biomol NMR, 2004 Oct;30(2):219-20.

Gelhaye E, Rouhier N, Gerard J, Jolivet Y, Gualberto J, Navrot N, Ohlsson PI, Wingsle G, Hirasawa M, Knaff DB, Wang H, Dizengremel P, Meyer Y, Jacquot JP. A specific form of thioredoxin h occurs in plant mitochondria and regulates the alternative oxidase. Proc Natl Acad Sci USA 2004, 101(40):14545-50.

Rouhier N, Gelhaye E, Jacquot JP. Plant glutaredoxins : still mysterious reducing systems. Cell. Mol. Life Sci. 2004, 61:1266-77.

Gelhaye E, Rouhier N, Jacquot JP. The thioredoxin h system of higher plants. Plant Physiol. Biochem. 2004, 42(4):265-71.


Rouhier N, Vlamis-Gardikas A, Lillig CH, Berndt C, Schwenn JD, Holmgren A, Jacquot JP. Characterization of the redox properties of poplar glutaredoxin. Antioxid. Redox Signal. 2003, 5(1):15-22.

Gelhaye E, Rouhier N, Vlamis-Gardikas A, Girardet JM, Sautière PE, Sauzet F, Martin F, Jacquot JP. Identification and characterization of a third thioredoxin h in poplar. Plant Physiol. Bioch. 2003, 41 : 629-635.

D’Ambrosio K, Kauffmann B, Rouhier N, Benedetti E, Jacquot JP, Aubry A, Corbier C. Crystallization and preliminary X-ray studies of the glutaredoxin from poplar in complex with glutathione. Acta Crystallogr. D Biol. Crystallogr. 2003, 59(Pt 6):1043-1045.

Rouhier N, Jacquot JP. Molecular and catalytic properties of a peroxiredoxin-glutaredoxin hybrid from Neisseria meningitidis. FEBS Lett. 2003, 554(1-2):149-53.


Rouhier N, Gelhaye E, Jacquot JP. Exploring the active site of plant glutaredoxin by site-directed mutagenesis. FEBS Lett. 2002, 511(1-3):145-9.

Rouhier N, Gelhaye E, Jacquot JP. Glutaredoxin-dependent peroxiredoxin from poplar: protein-protein interaction and catalytic mechanism. J. Biol. Chem. 2002, 277(16):13609-14.

Rouhier N, Gelhaye E, Sautiere PE, Jacquot JP. Enhancement of poplar glutaredoxin expression by optimization of the cDNA sequence. Prot. Expr. Purif. 2002, 24(2):234-41.

Gelhaye E, Rouhier N, Laurent P, Sautiere PE, Martin F, Jacquot JP. Isolation and characterization of an extended thioredoxin h from poplar.Physiol. Plant. 2002, 114(2):165-171.

Echalier A, Corbier C, Rouhier N, Jacquot JP, Aubry A. Crystallization and preliminary X-ray data of a bifunctional peroxiredoxin from poplar. Acta Crystallogr. D Biol. Crystallogr. 2002, 58(Pt 9):1501-3.

Rouhier N, Jacquot JP. Plant peroxiredoxins: alternative hydroperoxide scavenging enzymes. Photosynth. Res. 2002, 74: 259-268.

Jacquot JP, Gelhaye E, Rouhier N, Corbier C, Didierjean C, Aubry A. Thioredoxins and related proteins in photosynthetic organisms: molecular basis for thiol dependent regulation. Biochem. Pharmacol. 2002, 64(5-6):1065-9.

Jacquot JP, Rouhier N, Gelhaye E. Redox control by dithiol-disulfide exchange in plants: I. The chloroplastic systems. Ann. N Y Acad. Sci. 2002, 973:508-19.

Rouhier N, Gelhaye E, Jacquot JP. Redox control by dithiol-disulfide exchange in plants: II. The cytosolic and mitochondrial systems. Ann. N Y Acad. Sci. 2002, 973:520-8.


 Rouhier N, Gelhaye E, Sautiere PE, Brun A, Laurent P, Tagu D, Gerard J, de Fay E, Meyer Y, Jacquot JP. Isolation and characterization of a new peroxiredoxin from poplar sieve tubes that uses either glutaredoxin or thioredoxin as a proton donor. Plant Physiol. 2001, 127(3):1299-309